Evidence map›Paper›PMID 39847376›Full record

ArticleTranslational vision science & technology2025

Corneal Stromal Stem Cell-Derived Extracellular Vesicles Attenuate ANGPTL7 Expression in the Human Trabecular Meshwork.

Faycal Moujane, Chi Zhang, Robert Knight, John Y Lee, Sophie X Deng, Jie J Zheng

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Translational vision science & technology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Faycal MoujaneDepartment of Ophthalmology, Stein Eye Institute, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.
Chi ZhangDepartment of Ophthalmology, Stein Eye Institute, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.
Robert KnightDepartment of Ophthalmology, Stein Eye Institute, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.
John Y LeeDepartment of Ophthalmology, Stein Eye Institute, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.
Sophie X DengDepartment of Ophthalmology, Stein Eye Institute, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.
Jie J ZhengDepartment of Ophthalmology, Stein Eye Institute, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.

Funding

Vision Research Core at UCLAP30EY000331 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI DAVID S WILLIAMS · 1985 to 2026
$16.8M
Ex Vivo Expansion of Human Limbal Stem Cells for TransplantationR01EY021797 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Sophie Deng · 2012 to 2026
$5.1M
Development of small-molecule Wnt mimetics for corneal epithelial cell regenerationR01EY028557 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI DENG, SOPHIE, ZHENG, JIE J. · 2018 to 2021
$2.0M
NEI NIH HHS P30 EY000331NEI NIH HHS R01 EY021797NEI NIH HHS R01 EY028557
6 · The paper itself

Abstract

Purpose: Regulating intraocular pressure (IOP), mainly via the trabecular meshwork (TM), is critical in developing glaucoma. Whereas current treatments aim to lower IOP, directly targeting the dysfunctional TM tissue for therapeutic intervention has proven challenging. In our study, we utilized Dexamethasone (Dex)-treated TM cells as a model to investigate how extracellular vesicles (EVs) from immortalized corneal stromal stem cells (imCSSCs) could influence ANGPTL7 and MYOC genes expression within TM cells. Methods: Human TM cell lines were isolated and cultured from donor corneoscleral rims. EVs were purified from imCSSC conditioned media (CM) using size exclusion chromatography and characterized by nanoparticle tracking analysis, transmission electron microscopy (TEM), and ExoView technology. TM cells were treated with either Dex alone or with EVs for 5 days. Quantitative polymerase chain reaction (PCR) was carried out to quantify the mRNA level of MYOC and ANGPTL7. Results: A notable increase in the expression levels of MYOC and ANGPTL7 genes was observed compared with untreated TM cells (control). Furthermore, upon comparing Dex-treated TM cells with those receiving both Dex and EV treatments, a statistically significant reduction in ANGPTL7 expression (P < 0.05) was detected. Conclusions: The present study demonstrates that imCSSCs-derived EVs can effectively decrease the expression of ANGPLT7, a gene associated with fibrosis and implicated in the abnormal elevation of IOP in patients with glaucoma. Translational Relevance: Our study shows that imCSSC-derived EVs can specifically target ANGPTL7 expression, making them a promising preclinical therapy for glaucoma.

Indexed as

Angiopoietin-like ProteinsExtracellular VesiclesGene Expression RegulationStem CellsTrabecular MeshworkAngiopoietin-Like Protein 7Cells, CulturedCytoskeletal ProteinsDexamethasoneEye ProteinsGlycoproteinsHumansIntraocular PressureMyocilinAngiopoietin-Like Protein 7Angiopoietin-like ProteinsANGPTL7 protein, humanCytoskeletal ProteinsDexamethasoneEye ProteinsGlycoproteinsMyocilin

Identifiers

PMID39847376
PMCPMC11759583

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.