Evidence map›Paper›PMID 39847241›Full record

ArticleMolecular biology reports2025

Aberrant DNA methylation of EDNRB, MGMT and TIMP3 gene promoters in saliva of head and neck carcinoma patients as a diagnostic tool.

Jyoti Kanwar Shekhawat, Jyoti Sharma, Bikram Choudhury, Ankita Chugh, Purvi Purohit, Praveen Sharma, Mithu Banerjee

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Article in Molecular biology reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1 citing paper in PubMed.

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5 · Who and what money

Authors and funding

7 authors.

Jyoti Kanwar ShekhawatDepartment of Biochemistry, All India Institute of Medical Sciences, Jodhpur, Rajasthan, 342005, India.
Jyoti SharmaDepartment of Biochemistry, All India Institute of Medical Sciences, Jodhpur, Rajasthan, 342005, India.
Bikram ChoudhuryDepartment of Otorhinolaryngology, All India Institute of Medical Sciences, Jodhpur, Rajasthan, 342005, India.
Ankita ChughDepartment of Dentistry, All India Institute of Medical Sciences, Jodhpur, Rajasthan, India.
Purvi PurohitDepartment of Biochemistry, All India Institute of Medical Sciences, Jodhpur, Rajasthan, 342005, India.
Praveen SharmaDepartment of Biochemistry, All India Institute of Medical Sciences, Jodhpur, Rajasthan, 342005, India.
Mithu BanerjeeDepartment of Biochemistry, All India Institute of Medical Sciences, Jodhpur, Rajasthan, 342005, India. mithu.banerjee.3@gmail.com.

Funding

All India Institute of Medical Sciences, Jodhpur N/A
6 · The paper itself

Abstract

backgroundDifferential DNA methylation in the promoter region of tumour suppressor genes leads to gene function silencing. MATERIALS AND

methodsIn this study, we aimed to evaluate the salivary promoter methylation of EDNRB, MGMT and TIMP3 genes in H&NC patients (n = 100), premalignant lesions patients (n = 25) and healthy controls (n = 50). Blood and saliva samples were collected from all three groups and 20 concomitant tumour tissues were collected from the H&NC patients. Probe-based Methylation-specific PCR (MSP) was performed to assess the relative quantification of methylation.

resultsSignificant promoter hypermethylation was detected in all three genes between H&NC patients vs. healthy controls and premalignant lesion patients vs. healthy controls. Spearman correlation analysis showed, no significant association between methylation levels and clinicopathological characteristics of HNC patients while tobacco smoking was significantly related to EDNRB methylation in premalignant lesions. The receiver operating curve (ROC) generated for EDNRB, MGMT and TIMP3 genes from saliva samples was able to differentiate between cancer vs. healthy controls and premalignant vs. healthy controls. The combined diagnostic efficiency of the panel was higher than the genes singly. The combined sensitivity of EDNRB and TIMP3 increased to 92%.

conclusionThis indicates that EDNRB and TIMP3 have potential value in clinical practice as effective diagnostic markers for H&NC using saliva samples.

Indexed as

DNA MethylationDNA Modification MethylasesDNA Repair EnzymesHead and Neck NeoplasmsTissue Inhibitor of Metalloproteinase-3Tumor Suppressor ProteinsAdultAgedBiomarkers, TumorCase-Control StudiesFemaleHumansMaleMiddle AgedPromoter Regions, GeneticROC CurveBiomarkers, TumorDNA Modification MethylasesDNA Repair EnzymesMGMT protein, humanTIMP3 protein, humanTissue Inhibitor of Metalloproteinase-3Tumor Suppressor ProteinsBiomarkerHead and neck carcinomaMS-PCRSaliva

Identifiers

PMID39847241

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