Unlocking Probiotic Potential: Physicochemical Approaches to Evaluate Probiotic Bacterial Adhesion Potential to the Intestinal Tract.

Bacterial adhesion in the gut is critical to evaluate their effectiveness as probiotics. Understanding the bacterial adhesion within the complex gut environment is challenging. This study explores the adhesion mechanisms and the adhesion potential of five selected bacterial strains (Escherichia coli, Lactiplantibacillus plantarum, Faecalibacterium duncaniae, Bifidobacterium longum, and Bifidobacterium longum subsp. infantis) at the initial stages when bacterial cells arriving in the gut, using different physicochemical approaches. Bacterial morphology, rheology, and surface properties were evaluated. Surprisingly, previous methods such as bacterial adhesion to hydrocarbon and the interfacial tension between bacterial suspensions and mineral oil did not fully capture the bacterial adhesion to intestinal mucus. Consequently, this study introduced a novel approach to assess bacterial adhesion to mucus, based on contact angle measurements, calculation of surface tension, and work of adhesion. Interestingly, both small and large intestinal mucus are rather hydrophilic, and thus highly hydrophilic bacteria such as E. coli and B. infantis tend to adhere better. Additionally, a multicriteria evaluation of bacterial adhesion to the gut, from the bulk liquid transport stage until the irreversible adhesion, was proposed. E. coli and B. infantis demonstrated the highest overall adhesion potential in the intestinal tract, followed by Lpb. plantarum, B. longum, and F. duncaniae, respectively. This work contributed original physicochemical approaches to comprehensively examine bacterial adhesion in the gut.