Objective: To explore the combined uses of central vein isolation-based laparoscopic technique and tubeless cardiovascular interventional technique (CVIT) in laparoscopic adrenalectomy. Methods: 31 subject patients with adrenal tumors were recruited and treated from January 2020 to November 2021. Regarding tumor size, the average transverse diameter of the adrenal tumor was (2.2 ± 1.0) cm and the average longitudinal diameter of the tumor was (3.1 ± 1.5) cm, respectively. All subject patients were operated on through the abdominal approach. The "central vein isolation" based laparoscopic technique was adopted to complete the operation. No drainage tube was placed in the patients. For this study, selected performance parameters, including the operation time, intraoperative bleeding, postoperative hospital stays, and postoperative complications were recorded and analyzed. Results: All the tumors were removed laparoscopically without any conversion to open surgery. All 31 recruited subjects were treated successfully with preservation of adrenocortical function. The mean operation time was 30 min (range from 25 to 63 min); the mean amount of intraoperative bleeding was approximately 3 mL (ranges from 0 to 10 mL); the mean postoperative hospital stay was 3 days (range from 2 to 6 days). Of note, no complications were recorded, such as adjacent organ injury, large vessel injury, infection, and secondary bleeding that occurred during and after the operation. Conclusion: The combined use of central-vein isolation laparoscopic technique and tubeless treatment ensures a facile, safe, and robust laparoscopic adrenalectomy operation in clinical practice.