Evidence map›Paper›PMID 39844837›Full record

ArticleFrontiers in cellular and infection microbiology2024

Exosomes derived from syncytia induced by SARS-2-S promote the proliferation and metastasis of hepatocellular carcinoma cells.

Huilong Li, Haotian Lin, Tinghui Fan, Linfei Huang, Li Zhou, Xiaoyu Tian, Ruzhou Zhao, Yanhong Zhang, Xiaopan Yang, Luming Wan and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in cellular and infection microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Huilong Li *College of Basic Medical Sciences, School of Medicine, Zhejiang University, Hangzhou, China.
Haotian Lin *College of Basic Medical Sciences, School of Medicine, Zhejiang University, Hangzhou, China.
Tinghui Fan *Department of Genetic engineering, Beijing Institute of Biotechnology, Beijing, China.
Linfei Huang *Department of Genetic engineering, Beijing Institute of Biotechnology, Beijing, China.
Li ZhouDepartment of Genetic engineering, Beijing Institute of Biotechnology, Beijing, China.
Xiaoyu TianDepartment of Genetic engineering, Beijing Institute of Biotechnology, Beijing, China.
Ruzhou ZhaoDepartment of Genetic engineering, Beijing Institute of Biotechnology, Beijing, China.
Yanhong ZhangDepartment of Genetic engineering, Beijing Institute of Biotechnology, Beijing, China.
Xiaopan YangDepartment of Genetic engineering, Beijing Institute of Biotechnology, Beijing, China.
Luming WanDepartment of Genetic engineering, Beijing Institute of Biotechnology, Beijing, China.
Hui ZhongDepartment of Genetic engineering, Beijing Institute of Biotechnology, Beijing, China.
Nan JiangDepartment of Pharmacy, Medical Supplies Center of People's Liberation Army (PLA) General Hospital, Beijing, China.
Congwen WeiDepartment of Genetic engineering, Beijing Institute of Biotechnology, Beijing, China.
Wei ChenCollege of Basic Medical Sciences, School of Medicine, Zhejiang University, Hangzhou, China.
Lihua HouCollege of Basic Medical Sciences, School of Medicine, Zhejiang University, Hangzhou, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Coronavirus disease 2019 (COVID-19) is characterized by fever, fatigue, dry cough, dyspnea, mild pneumonia and acute lung injury (ALI), which can lead to acute respiratory distress syndrome (ARDS), and SARS-CoV-2 can accelerate tumor progression. However, the molecular mechanism for the increased mortality in cancer patients infected with COVID-19 is unclear. Methods: Colony formation and wound healing assays were performed on Huh-7 cells cocultured with syncytia. Exosomes were purified from the cell supernatant and verified by nanoparticle tracking analysis (NTA), Western blot (WB) analysis and scanning electron microscopy (SEM). Differentially expressed proteins in syncytia-derived exosomes (Syn-Exos) and their functions was analyzed by Proteomic sequencing. Syn-Exo-mediated promotion of hepatocellular carcinoma cells was measured by CCK-8 and Transwell migration assays. The mechanism by which Syn-Exos promote tumor growth was analyzed by Western blotting. A patient-derived xenotransplantation (PDX) mouse model was constructed to evaluate the pathological role of the SARS-CoV-2 spike protein (SARS-2-S). The number of syncytia in the tumor tissue sections was determined by immunofluorescence analysis. Results: Syncytium formation promoted the proliferation and migration of hepatocellular carcinoma cells. Proteomic sequencing revealed that proteins that regulate cell proliferation and metastasis in Syn-Exos were significantly upregulated. Syn-Exos promote the proliferation and migration of hepatocellular carcinoma cells. Animal experiments showed that a pseudotyped lentivirus bearing SARS-2-S (SARS-2-Spp) promoted tumor development in PDX mice. More syncytia were found in tumor tissue from SARS-2-Spp mice than from VSV-Gpp mice. Conclusions: Syn-Exos induced by SARS-2-S can promote the proliferation and metastasis of hepatocellular carcinoma cells.

Indexed as

Carcinoma, HepatocellularCOVID-19ExosomesGiant CellsLiver NeoplasmsSARS-CoV-2Spike Glycoprotein, CoronavirusAnimalsCell Line, TumorCell MovementCell ProliferationHumansMiceNeoplasm MetastasisSpike Glycoprotein, Coronavirusexosomeshepatocellular carcinomametastasisproliferationSARS-2-Ssyncytia

Identifiers

PMID39844837
PMCPMC11750861

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.