ArticleGenes and environment : the official journal of the Japanese Environmental Mutagen Society2025
Diurnal and daily fluctuations in levels of the urinary oxidative stress marker 8-hydroxyguanosine in spot urine samples.
Article in Genes and environment : the official journal of the Japanese Environmental Mutagen Society, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundUrinary 8-hydroxyguanosine (8-OHGuo) levels serve as a biomarker for oxidative stress and hydroxyl radical-induced RNA damage. Evaluating the diurnal and daily fluctuations in urinary 8-OHGuo excretion levels is essential for understanding its implications. However, research in this area remains limited. In this study, we aim to investigate the diurnal and daily fluctuations in 8-OHGuo levels as well as the factors that influence these variations, using spot urine samples.
methodsUrine samples were collected from seven healthy participants during each urination from the time of awakening until 24:00 h to evaluate diurnal variations. To assess daily fluctuations, urine samples were collected from 18 healthy participants at the time of awakening for 23 consecutive days. The urinary 8-OHGuo levels were measured using an HPLC-ECD method.
resultsNo significant variations were observed in the diurnal levels of urinary 8-OHGuo among non-smokers. Conversely, the daily variation of 8-OHGuo in the urine of the smoker was significant, with a coefficient of variation of 18.71%. Each individual maintained a characteristic value despite some diurnal fluctuations. Furthermore, the daily levels of 8-OHGuo exhibited a range of variations influenced by lifestyle factors, including mental state, sleep duration, smoking, menstrual cycle, and dietary habits.
conclusionAs a specific marker of RNA oxidation, 8-OHGuo provides unique insights distinct from those provided by the widely used DNA oxidation marker 8-hydroxydeoxyguanosine as an indicator of oxidative stress. Urinary 8-OHGuo could serve as a valuable biomarker for managing and preventing oxidative stress-related diseases, provided that the specific range of daily variations is established. The high daily variation in urinary 8-OHGuo levels necessitates the use of multiple samples to accurately determine individual levels. However, further research with large sample sizes will help to validate these findings.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.