Evidence map›Paper›PMID 39844202›Full record

ArticleBMC oral health2025

Tensile force promotes osteogenic differentiation via ephrinB2-EphB4 signaling pathway in orthodontic tooth movement.

Hang Yu, Xiaoxi Wei, Huan Jiang, Huichuan Qi, Yi Zhang, Min Hu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC oral health, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Hang YuDepartment of Orthodontics, Stomatology School of Jilin University, No. 1500 Qinghua Road, ChaoYang Area, Changchun City, Jilin Province, P.R. China.
Xiaoxi WeiDepartment of Orthodontics, Stomatology School of Jilin University, No. 1500 Qinghua Road, ChaoYang Area, Changchun City, Jilin Province, P.R. China.
Huan JiangDepartment of Orthodontics, Stomatology School of Jilin University, No. 1500 Qinghua Road, ChaoYang Area, Changchun City, Jilin Province, P.R. China.
Huichuan QiDepartment of Orthodontics, Stomatology School of Jilin University, No. 1500 Qinghua Road, ChaoYang Area, Changchun City, Jilin Province, P.R. China.
Yi ZhangDepartment of Orthodontics, Stomatology School of Jilin University, No. 1500 Qinghua Road, ChaoYang Area, Changchun City, Jilin Province, P.R. China. zhangyi0519@jlu.edu.cn.
Min HuDepartment of Orthodontics, Stomatology School of Jilin University, No. 1500 Qinghua Road, ChaoYang Area, Changchun City, Jilin Province, P.R. China. humin@jlu.edu.cn.

Funding

Finance department Project of Jilin Province in China Nos. JCSZ2023481-35National Natural Science Foundation of China 81801005
6 · The paper itself

Abstract

objectiveTo investigating whether osteogenic differentiation of osteoblasts promoted by tension force (TF) is mediated by ephrinB2-EphB4 signaling.

methodsTF was applied to MC3T3-E1 cells, then CCK-8 and live/dead staining were used to detect cell proliferation. Levels of osteogenic differentiation-related factors were detected by ALP staining, ARS staining, qPCR and western blot. NVP-BHG712 was used to block EphB4 receptor. Establishing a rat orthodontic tooth movement (OTM) model, ephrinB2-Fc and NVP-BHG712 were used to treat rats. Micro-CT and H&E staining were used to detect alveolar bone. Changes of MAPK pathways were detected to investigate whether they were downstream of ephrinB2-EphB4 signaling in mediating TF promote osteogenic differentiation.

resultWe explored the effect of TF on MC3T3-E1 cells, and found that TF significantly promoted osteogenic differentiation, but when EphB4 receptor was blocked, the promotion was inhibited. In vivo, we found that TF improved alveolar bone formation through ephrinB2-EphB4 signaling. Further investigation into the signaling pathways revealed that TF significantly increased levels of MAPK pathways, however, when EphB4 receptor was blocked, only the promotion of p-ERK1/2 was decreased.

conclusionTF promotes osteogenic differentiation through ephrinB2-EphB4 signaling and ERK1/2 pathway is a downstream of ephrinB2-EphB4 signaling partially mediate mediates TF-induced promotion of osteogenic differentiation.

Indexed as

Cell DifferentiationEphrin-B2OsteogenesisReceptor, EphB4Signal TransductionTensile StrengthToothAnimalsCell LineCell ProliferationMaleMiceRatsRats, Sprague-DawleyEphb4 protein, mouseEphb4 protein, ratEphrin-B2Receptor, EphB4ephrinB2-EphB4ERK1/2Orthodontic tooth movementOsteogenic differentiationTensile force

Identifiers

PMID39844202
PMCPMC11755856

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.