Evidence map›Paper›PMID 39844195›Full record

ArticleJournal of biomedical science2025

Temporal and spatial pattern of DNA damage in neurons following spinal cord Injury in mice.

Elle Em Scheijen, Naomi Veeningen, Sam Duwé, Anna Ivanova, Jana Van Broeckhoven, Sven Hendrix, David M Wilson

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of biomedical science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. NADSpinal cord · 2026
    Review
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Elle Em ScheijenNeurosciences, Biomedical Research Institute, Hasselt University, Agoralaan Building C, 3590, Diepenbeek, Belgium.
Naomi VeeningenImmunology and Infection, Biomedical Research Institute, Hasselt University, Agoralaan Building C, 3590, Diepenbeek, Belgium.
Sam DuwéAdvanced Optical Microscopy Centre, Biomedical Research Institute, Hasselt University, Agoralaan Building C, 3590, Diepenbeek, Belgium.
Anna IvanovaData Science Institute, Biomedical Research Institute, Hasselt University, Agoralaan Building D, 3590, Diepenbeek, Belgium.
Jana Van BroeckhovenImmunology and Infection, Biomedical Research Institute, Hasselt University, Agoralaan Building C, 3590, Diepenbeek, Belgium.
Sven HendrixInstitute for Translational Medicine, Medical School Hamburg, Am Kaiserkai 1, 20457, Hamburg, Germany.
David M WilsonNeurosciences, Biomedical Research Institute, Hasselt University, Agoralaan Building C, 3590, Diepenbeek, Belgium. david.wilson@uhasselt.be.ORCID http://orcid.org/0000-0002-8945-0395

Funding

Fonds Wetenschappelijk Onderzoek G0H3716NFonds Wetenschappelijk Onderzoek GOC2120FWOUniversiteit Hasselt BOF20DOC16
6 · The paper itself

Abstract

backgroundDeficient DNA repair and excessive DNA damage contribute to neurodegenerative disease. However, the role of DNA damage and repair in spinal cord injury (SCI) is unclear. SCI, a debilitating disruption of the structural and biological network of the spinal cord, is characterized by oxidative stress. Nevertheless, the pathophysiological mechanisms leading to neuronal loss following SCI remain incompletely defined.

methodsUsing a contusion model, a severe SCI was induced at the L1 spinal level in C57Bl/6J mice. The temporal and spatial presence of DNA damage was then determined via immunolabeling for the DNA damage marker, γH2AX, from 1 h post-injury (hpi) to 28 days post-injury (dpi).

resultsOur analysis revealed that increased DNA damage foci were present from 1 hpi to 3 dpi in SCI mice relative to controls (sham surgery and naive), with the damage signal spreading over time longitudinally from the affected area to more rostral and caudal regions. Co-labeling of γH2AX with NeuN revealed neuronal specificity of DNA damage, with increased early cell death (pan-nuclear γH2AX) peaking at 1 dpi and apoptosis (cleaved Caspase-3) arising later at 3 dpi.

conclusionOur study indicates a possible role of DNA damage in neuronal loss following SCI and highlights the need for early interventions targeting DNA repair to preserve neuronal tissue.

Indexed as

DNA DamageNeuronsSpinal Cord InjuriesAnimalsDNA RepairFemaleHistonesMaleMiceMice, Inbred C57BLHistonesDNA damageDNA repairGamma-H2AXNeuronal deathOxidative stressSpinal cord injury

Identifiers

PMID39844195
PMCPMC11756142

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.