ArticleBMC genomics2025
Proteomics and metabolomics analyses of mechanism underlying bovine sperm cryoinjury.
Article in BMC genomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Ultra-Low Temperature Cryopreservation of Sperm from a New Type of Hybrid Bream.International journal of molecular sciences · 2026Article
- LCAT acts as a molecular switch and regulates rooster sperm motility in vitro.Molecular & cellular proteomics : MCP · 2026Article
- Article
- Metabolomics and Lipidomics in the Study of Reproductive Performance in Livestock.Animals : an open access journal from MDPI · 2026Review
- Ginseng Peptide Improves the Cryopreservation Efficiency and Fertilization Potential of Yak Semen via FOXO1/PI3K/AKT Axis.Antioxidants (Basel, Switzerland) · 2026Article
- Metabolomic profiling of goat seminal plasma: insights into sperm motility regulation.BMC veterinary research · 2026Article
- Deep learning-enabled morphology analysis of bovine sperm for label-free imaging flow cytometry.Frontiers in veterinary science · 2026Article
- Integrated Proteomics and Metabolomics Reveal Spermine Enhances Sperm Freezability via Antioxidant Pathways.Antioxidants (Basel, Switzerland) · 2025Article
- Optimization of Vitrification Protocols for Feline Epididymal Spermatozoa: Impact on Post-Warming Sperm Quality and Fertilizing Potential.Animals : an open access journal from MDPI · 2025Article
- Effect ofAnimals : an open access journal from MDPI · 2025Article
- Relation between proteome characterization and semen quality in Italian chicken breeds.PloS one · 2025Article
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Authors and funding
10 authors.
Funding
Abstract
backgroundThe cryoinjury of semen during cryopreservation reduces sperm motility, constraining the application of artificial insemination (AI) in bovine reproduction. Some fertility markers, related to sperm motility before and after freezing have been identified. However, little is known about the biological mechanism through which freezing reduces sperm motility. This study investigated the selective effects of cryoinjury on high-motility sperm (HMS) and low-motility sperm (LMS) in frozen-thawed from the perspectives of reactive oxygen species (ROS), mitochondrial membrane potential (MMP), and ATP levels. The molecular mechanism of decreased sperm motility caused by cryoinjury was explored through a joint analysis of 4D-label free quantitative proteomics and non-targeted metabolomics.
resultsThe results indicate that low levels of ROS and high degrees of MMP and ATP play a critical role in the survival of HMS during the freezing process. The sperm samples from the frozen-thawed HMS and LMS were analysed for proteomics and metabolomics, 2,465 proteins and 4,135 metabolites were detected in bovine sperm samples. In contrast to LMS, HMS have 106 proteins and 106 metabolites with high abundance expression, and 79 proteins and 223 metabolites with low abundance expression. Proteomics and metabolomics data exhibit that highly expressed antioxidant enzymes and metabolites in HMS can maintain sperm motility by regulating the ROS produced during freezing to prevent sperm from oxidative stress and apoptosis. Furthermore, the KEGG analysis of differential proteins and metabolites during the freezing process implies that the significant enrichment of glycolysis and cAMP in HMS can guarantee energy supply.
conclusionsThe results provided that during the process of bovine sperm freezing, highly expressed antioxidant enzymes can regulate the reactive oxygen species levels to avoid oxidative stress and the glycolysis signalling pathway ensures ATP production can sustain frozen-thawed sperm motility.
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