Evidence map›Paper›PMID 39843625›Full record

ArticleScientific reports2025

Unraveling the distinctive gut microbiome of khulans (Equus hemionus hemionus) in comparison to their drinking water and closely related equids.

Víctor Hugo Jarquín-Díaz, Anisha Dayaram, Eirini S Soilemetzidou, Amelie Desvars-Larrive, Julia Bohner, Bayarbaatar Buuveibaatar, Petra Kaczensky, Chris Walzer, Alex D Greenwood, Ulrike Löber

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Víctor Hugo Jarquín-Díaz *Department of Wildlife Diseases, Leibniz Institute for Zoo and Wildlife Research, Alfred- Kowalke Str. 17, 10315, Berlin, Germany.
Anisha Dayaram *Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.
Eirini S Soilemetzidou *Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.
Amelie Desvars-LarriveResearch Institute of Wildlife Ecology, University of Veterinary Medicine, Vienna, Austria.
Julia BohnerMax-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.
Bayarbaatar BuuveibaatarWildlife Conservation Society, Mongolia Program, Ulaanbaatar, Mongolia.
Petra KaczenskyResearch Institute of Wildlife Ecology, University of Veterinary Medicine, Vienna, Austria.
Chris WalzerResearch Institute of Wildlife Ecology, University of Veterinary Medicine, Vienna, Austria.
Alex D GreenwoodMax-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany. greenwood@izw-berlin.de.
Ulrike LöberDepartment of Wildlife Diseases, Leibniz Institute for Zoo and Wildlife Research, Alfred- Kowalke Str. 17, 10315, Berlin, Germany. Ulrike.Loeber@mdc-berlin.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The microbial composition of host-associated microbiomes is influenced by co-evolutionary interactions, host genetics, domestication, and the environment. This study investigates the contribution of environmental microbiota from freshwater bodies to the gastrointestinal microbiomes of wild khulans (Equus hemionus hemionus, n = 21) and compares them with those of captive khulans (n = 12) and other equids-Przewalski's horse (n = 82) and domestic horse (n = 26). Using PacBio technology and the LotuS pipeline for 16S rRNA gene sequencing, we analyze microbial diversity and conduct differential abundance, alpha, and beta diversity analyses. Results indicate limited microbial sharing between wild khulans and their waterhole environments, suggesting minimal environmental influence on their gut microbiomes and low levels of water contamination by khulans. Wild khulans exhibit greater microbial diversity and richness compared to captive ones, likely due to adaptations to the harsh nutritional conditions of the Gobi desert. Conversely, captive khulans show reduced microbial diversity, potentially affected by dietary changes during captivity. These findings highlight the significant impact of environment and lifestyle on the gut microbiomes of equids.

Indexed as

Drinking WaterEquidaeGastrointestinal MicrobiomeAnimalsBacteriaHorsesRNA, Ribosomal, 16SDrinking WaterRNA, Ribosomal, 16S16S rRNA full length gene sequencingEquus hemionus hemionusFreshwater microbiotaGobi DesertMicrobiomePacBio

Identifiers

PMID39843625
PMCPMC11754619

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