Evidence map›Paper›PMID 39843481›Full record

ArticleScientific reports2025

Multiplex-PCR technique to predict polymorphic antigens - M, N, S and s - and associations between their alleles and Mi

Thanaporn Kerdthip, Oytip Nathalang, Sarisa Chidtrakoon, Tanaporn Choychimplee, Dollapak Apipongrat, Kamphon Intharanut

Abstract read
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Thanaporn KerdthipGraduate Program in Medical Technology, Faculty of Allied Health Sciences, Thammasat University, Pathumthani, 12120, Thailand.
Oytip NathalangGraduate Program in Medical Technology, Faculty of Allied Health Sciences, Thammasat University, Pathumthani, 12120, Thailand.
Sarisa ChidtrakoonBlood Bank, Thammasat University Hospital, Pathumtani, Thailand.
Tanaporn ChoychimpleeGraduate Program in Medical Technology, Faculty of Allied Health Sciences, Thammasat University, Pathumthani, 12120, Thailand.
Dollapak ApipongratDivision of Hematology, Department of Medicine, Phramongkutklao Hospital, Bangkok, Thailand.
Kamphon IntharanutGraduate Program in Medical Technology, Faculty of Allied Health Sciences, Thammasat University, Pathumthani, 12120, Thailand. kamphon.int@gmail.com.

Funding

Thammasat University Research Fund TUFT 80/2567
6 · The paper itself

Abstract

Serological typing of MNS polymorphic antigens - M, N, S and s - remains a fundamental technique in transfusion medicine and prenatal care, providing essential information for matching blood donors and recipients and managing haemolytic disease. Although this method is well proven and routinely used, it is not a comprehensive solution, as it has several weaknesses. Alternatively, multiplex polymerase chain reaction (PCR) is a commonly used genotyping tool due to its potency and ability to amplify several DNA targets simultaneously in a single reaction. In this work, we aimed to develop multiplex PCR and evaluate its performance for GYPA*M, GYPA*N, GYPB*S, and GYPB*s allele identification using serological and DNA sequencing methods. We also aimed to investigate the correlation between these alleles and Mi

Indexed as

GlycophorinsMNSs Blood-Group SystemMultiplex Polymerase Chain ReactionAllelesBlood Grouping and CrossmatchingGenotypeHumansSequence Analysis, DNAGlycophorinsGYPB protein, humanMNSs Blood-Group SystemGenetic variabilityMia-associated hybrid glycophorinsMNS blood group systemMultiplex PCRRed cell genotyping

Identifiers

PMID39843481
PMCPMC11754802

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.