Evidence map›Paper›PMID 39843433›Full record

ArticleNature communications2025

Multichrome encoding-based multiplexed, spatially resolved imaging reveals single-cell RNA epigenetic modifications heterogeneity.

Dongsheng Mao, Xiaochen Tang, Runchi Zhang, Song Hu, Hongquan Gou, Penghui Zhang, Wenxing Li, Qiuhui Pan, Bing Shen, Xiaoli Zhu

Abstract read
In one paragraph

Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Dongsheng Mao *Shanghai Tenth People's Hospital of Tongji University, Shanghai, PR China.ORCID http://orcid.org/0000-0002-3881-0418
Xiaochen Tang *Department of Clinical Laboratory Medicine, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai, PR China.
Runchi ZhangShanghai Tenth People's Hospital of Tongji University, Shanghai, PR China.
Song HuShanghai Pudong New Area People's Hospital, Shanghai, PR China.
Hongquan GouShanghai Tenth People's Hospital of Tongji University, Shanghai, PR China.
Penghui ZhangShanghai Pudong New Area People's Hospital, Shanghai, PR China.
Wenxing LiShanghai Tenth People's Hospital of Tongji University, Shanghai, PR China. wenxingapp@163.com.ORCID http://orcid.org/0009-0001-2536-8878
Qiuhui PanDepartment of Clinical Laboratory Medicine, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai, PR China. panqiuhui_med@163.com.ORCID http://orcid.org/0000-0001-7386-2590
Bing ShenShanghai Tenth People's Hospital of Tongji University, Shanghai, PR China. urodrshenbing@shsmu.edu.cn.ORCID http://orcid.org/0000-0002-0952-2257
Xiaoli ZhuShanghai Tenth People's Hospital of Tongji University, Shanghai, PR China. xiaolizhu@tongji.edu.cn.ORCID http://orcid.org/0000-0001-5497-4538

Funding

National Natural Science Foundation of China (National Science Foundation of China) 32301256National Natural Science Foundation of China (National Science Foundation of China) 32371531National Natural Science Foundation of China (National Science Foundation of China) 82102477National Natural Science Foundation of China (National Science Foundation of China) 82172357National Natural Science Foundation of China (National Science Foundation of China) 82293662Natural Science Foundation of Shanghai (Natural Science Foundation of Shanghai Municipality) 23ZR1449100Shanghai Municipal Health Bureau (Shanghai Municipal Public Health Bureau) 20234Y0005
6 · The paper itself

Abstract

Understanding the heterogeneity of epigenetic modifications within single cells is pivotal for unraveling the nature of the complexity of gene expression and cellular function. In this study, we have developed a strategy based on multichrome encoding and "AND" Boolean logic recognition for multiplexed, spatially resolved imaging of single-cell RNA epigenetic modifications, termed as PRoximity Exchange-assisted Encoding of Multichrome (PREEM). Through the implementation of this strategy, we can now map the expression and nuclear distribution of multiple site-specific RNA N6-methyladenosine (m

Indexed as

Epigenesis, GeneticRNASingle-Cell AnalysisAdenosineHumansSingle Molecule ImagingAdenosineN-methyladenosineRNA

Identifiers

PMID39843433
PMCPMC11754832

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.