ArticleThe Journal of veterinary medical science2025
Evaluation of storage conditions and periods for a three-drug anesthetic mixture in mice.
Article in The Journal of veterinary medical science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
The three-drug anesthetic mixture (medetomidine, midazolam and butorphanol), developed as an injectable anesthetic for laboratory animals, has been verified from various perspectives and applied to mice and other laboratory animals. However, the effects of its storage conditions and periods on its efficacy have not yet been studied. This study investigated the mixture's efficacy after storage under various conditions (room temperature, 4°C and -20°C) for 1 and 2 years. Mice in all groups were induced into a stable anesthetic state for at least 15 min after administration. The mice recovered from the anesthetized state 35 min after administration of the antagonist. These findings demonstrate the mixture's stability under different storage conditions and durations, potentially improving laboratory mouse welfare.
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