ArticleScience advances2025
Creating coveted bioluminescence colors for simultaneous multi-color bioimaging.
Article in Science advances, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed.
- An Open, Integrated Platform for Multiplexed Bioluminescence Microscopy.Chemical & biomedical imaging · 2026Article
- PrismaLuc luciferase enables tunable near-infrared bioluminescence forRSC chemical biology · 2026Article
- Sweet and Bright: Illuminating Glycoprotein-Mediated Endocytosis via Metabolic Labeling and NanoLuciferase.ACS chemical biology · 2026Article
- Expanded applications of bioluminescence microscopy with phasor analysis.Cell reports methods · 2026Article
- A Modular Platform for Quantitative Two-Color Bioluminescence Combining NanoLuc and Red-Shifted NanoPrism Luciferases.ACS chemical biology · 2026Article
- Continuous Hypermutation and Evolution of Luciferase Variants.ACS chemical biology · 2026Article
- Engineering glowing plants: recent progress and future directions for application-oriented design.Frontiers in bioengineering and biotechnology · 2026Review
- Autonomous Bioluminescence Systems: From Molecular Mechanisms to Emerging Applications.JACS Au · 2025Review
- The latest developments of near-infrared fluorescent probes from NIR-I to NIR-II for bioimaging.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bioluminescence, an optical marker that does not require excitation by light, allows researchers to simultaneously observe multiple targets, each exhibiting a different color. Notably, the colors of the bioluminescent proteins must sufficiently vary to enable simultaneous detection. Here, we aimed to introduce a method that can be used to expand the color variation by tuning dual-acceptor bioluminescence resonance energy transfer. Using this approach, we could visualize multiple targets with up to 20 colors through single-shot acquisition using a color complementary metal-oxide semiconductor camera. Overall, this method enables simple and simultaneous observation of multiple biological targets and phenomena.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.