Evidence map›Paper›PMID 39841309›Full record

ArticleMolecular biology reports2025

Rapid detection assays for Bacillus anthracis, Yersinia pestis, and Brucella spp. via triplex-recombinase polymerase amplification.

Meijie Feng, Jinping Ma, Yan Zhang, Dongshu Wang, Li Zhu, Chao Pan, Hengliang Wang, Xiankai Liu, Yuanzhi Wang, Ying Meng and 1 more

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Article in Molecular biology reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

11 authors.

Meijie Feng *College of Food Science and Technology, Shanghai Ocean University, 999 Hucheng Huan Road, Lingang New City, Shanghai, 201306, China.
Jinping Ma *Wuzhong People's Hospital, 143 Xinmin Road, Litong District, Wuzhong City, Ningxia Hui Autonomous Region, 751100, China.
Yan Zhang *College of Food Science and Technology, Shanghai Ocean University, 999 Hucheng Huan Road, Lingang New City, Shanghai, 201306, China.
Dongshu WangState Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Biotechnology, 20 Dongdajie Street, Fengtai District, Beijing, 100071, China.
Li ZhuState Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Biotechnology, 20 Dongdajie Street, Fengtai District, Beijing, 100071, China.
Chao PanState Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Biotechnology, 20 Dongdajie Street, Fengtai District, Beijing, 100071, China.
Hengliang WangCollege of Food Science and Technology, Shanghai Ocean University, 999 Hucheng Huan Road, Lingang New City, Shanghai, 201306, China.
Xiankai LiuState Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Biotechnology, 20 Dongdajie Street, Fengtai District, Beijing, 100071, China.
Yuanzhi WangSchool of Medicine, Shihezi University, Shihezi, Xinjiang Uygur Autonomous Region, 832002, China. wangyuanzhi621@126.com.
Ying MengTongliao Center of Disease Control and Prevention, Keerqin District, Tongliao City, Inner Mongolia Autonomous Region, 028005, China. tlmy0331@163.com.
Yufei LyuState Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Biotechnology, 20 Dongdajie Street, Fengtai District, Beijing, 100071, China. flygogo.cool@163.com.

Funding

National Key R&D Program of China 2022YFC2304003National Natural Science Foundation of China 82102412,82172317
6 · The paper itself

Abstract

backgroundBacillus anthracis (B. anthracis), Yersinia pestis (Y. pestis), and Brucella spp. are zoonotic bacteria that cause anthrax, plague, and brucellosis, respectively. Outbreaks typically occur in remote regions with poor transportation and limited laboratory testing. Therefore, a simple, sensitive, multiplex nucleic acid detection method is essential for effective disease management and control.

methodsPrimers and probes for the three pathogens were designed to reduce interference from related strains. Three recombinase polymerase amplification (RPA) reactions were conducted at 39 °C for 10 min to produce species-specific fluorescence signals for the three pathogens. These were integrated, and conditions were optimized for rapid, sensitive triplex-RPA assays without cross-reactivity. A triplex-RPA reaction with lateral flow dipsticks (LFDs) was developed and applied to blood samples, newly isolated strains, and simulated samples.

resultsHighly sensitive and specific primers and probes were developed, achieving a maximum sensitivity of 1 copy/µL in single-reaction RPA. The optimized triplex RPA detection technique, combined with fluorescence, effectively identified B. anthracis, Y. pestis, and Brucella spp. within 20 min, whereas LFDs achieved detection in 10 min. The assay also performed comparably to conventional polymerase chain reaction techniques when tested on blood samples, newly isolated strains, and simulated samples.

conclusionsThis study offers reliable methods for detecting B. anthracis, Y. pestis, and Brucella spp. in rural hospitals and public health initiatives.

Indexed as

Bacillus anthracisBrucellaNucleic Acid Amplification TechniquesYersinia pestisAnthraxBrucellosisDNA PrimersHumansMultiplex Polymerase Chain ReactionPlagueRecombinasesSensitivity and SpecificityDNA PrimersRecombinasesBacillus anthracisBrucella spp.Lateral flow dipsticksRecombinase polymerase amplificationTriplex-RPAYersinia pestis

Identifiers

PMID39841309

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