Evidence map›Paper›PMID 39841186›Full record

ArticleProtoplasma2025

Successful application of microspore culture by adding kinetin and methylene blue to improve microspore embryogenesis in Chinese kale breeding.

Haonan Li, Zhe Wang, Weina Zhou, Jinyan Li, Zifan Zhao, Chao Zheng, Zhiyong Liu, Yun Zhang

Abstract read
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In one paragraph

Article in Protoplasma, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Haonan LiCollege of Horticulture, Shenyang Agricultural University, Shenhe District, 120 Dongling Road, Shenyang, China.
Zhe WangCollege of Horticulture, Shenyang Agricultural University, Shenhe District, 120 Dongling Road, Shenyang, China.
Weina ZhouCollege of Horticulture, Shenyang Agricultural University, Shenhe District, 120 Dongling Road, Shenyang, China.
Jinyan LiCollege of Horticulture, Shenyang Agricultural University, Shenhe District, 120 Dongling Road, Shenyang, China.
Zifan ZhaoCollege of Horticulture, Shenyang Agricultural University, Shenhe District, 120 Dongling Road, Shenyang, China.
Chao ZhengCollege of Horticulture, Shenyang Agricultural University, Shenhe District, 120 Dongling Road, Shenyang, China.
Zhiyong LiuCollege of Horticulture, Shenyang Agricultural University, Shenhe District, 120 Dongling Road, Shenyang, China.
Yun ZhangCollege of Horticulture, Shenyang Agricultural University, Shenhe District, 120 Dongling Road, Shenyang, China. zhangyun511@syau.edu.cn.

Funding

basic scientific research project of educational department of Liaoning JYTYB2024055
6 · The paper itself

Abstract

Microspore culture is an efficient and rapid method that produces doubled haploid (DH) lines for hybrid breeding in crops and vegetables. However, the low frequency of microspore embryogenesis and spontaneous diploidization in Chinese kale still require improvement. In the present work, an efficient microspore culture protocol was constructed and used for DH producing in Chinese kale breeding. The results showed that kinetin (KT) and methylene blue (MB) improved microspore embryogenesis and embryo development. The spontaneous diploidization rates were below 14.48%. Therefore, artificial doubling was used for haploids; the doubled haploid rate was significantly increased, and the highest doubling rate reached 33.75% with pendimethalin. 98 DH lines were obtained in this study. This study also identified the horticultural characters of the DH lines, which showed significant differences between them. All DH lines were self-incompatible. The hybrid crosses were performed using five DH lines selected for the Chinese kale breeding. This study was helpful to accelerate the application of microspore culture and provide DH lines for Chinese kale hybrid breeding.

Indexed as

BrassicaKinetinMethylene BluePlant BreedingPollenHaploidyKinetinMethylene BlueBreedingChinese kaleChromosome doublingDoubled haploid linesMicrospore embryogenesis

Identifiers

PMID39841186

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.