Evidence map›Paper›PMID 39839716›Full record

ArticlemicroPublication biology2025

Improved RNA toehold switch sensitivity using the NanoLuc complementation reporter.

Katherinne J Diaz, Julian Jarquin, Aleksandr Petrosyan, Melissa K Takahashi

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in microPublication biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Synthetic toehold biosensors for detectingFrontiers in microbiology · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Katherinne J DiazBiology Department, California State University, Northridge, Northridge, California, United States.
Julian JarquinBiology Department, California State University, Northridge, Northridge, California, United States.
Aleksandr PetrosyanBiology Department, California State University, Northridge, Northridge, California, United States.
Melissa K TakahashiBiology Department, California State University, Northridge, Northridge, California, United States.

Funding

Discovering novel antimicrobial agents that target sRNA regulated antibiotic resistance mechanismsSC2GM136500 · NIGMS · CALIFORNIA STATE UNIVERSITY NORTHRIDGE · PI TAKAHASHI, MELISSA K. · 2020 to 2022
$435k
NIGMS NIH HHS SC2 GM136500
6 · The paper itself

Abstract

RNA toehold switches are powerful tools that can be used as biosensors to detect nearly any RNA sequence. In the presence of a specific RNA trigger, the toehold switch allows translation of a reporter protein. Toehold switches expressed in cell-free expression systems have been used as biosensors for several viruses and bacterial RNAs. Here we explore the use of the NanoLuc complementation reporter NanoBiT and found that it provides improved sensitivity over sfGFP. This reporter can be applied broadly across other RNA toehold switch applications.

Identifiers

PMID39839716
PMCPMC11749064

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.