ArticleSmall (Weinheim an der Bergstrasse, Germany)2025
Capturing Conformational Transitions of Fluorescently-Coupled Polyelectrolyte Brushes with High Spatiotemporal Resolution.
Article in Small (Weinheim an der Bergstrasse, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Responsive Polyelectrolyte Brushes in Applications: Functions, Stimuli, and Design Considerations.Advanced materials (Deerfield Beach, Fla.) · 2025Review
- Capturing Conformational Transitions of Fluorescently-Coupled Polyelectrolyte Brushes with High Spatiotemporal Resolution.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Polyelectrolyte brushes (PEBs) undergo conformational transitions due to changes in pH and/or ionic strength, which is leveraged as smart surfaces and on-demand drug-release systems. However, probing conformational transitions of functional PEBs has remained challenging due to low spatiotemporal resolution of characterization methods. Herein, fluorescently-coupled PEBs are devised that give rise to Förster Resonance Energy Transfer (FRET) intrinsically coupled to conformational transitions of chains. Polyelectrolyte poly(2-(dimethylamino)ethyl methacrylate) brushes are grown on silica surfaces via a grafting-from approach, producing nanoscale brushes ≈60 nm in solvated height. The study chose to investigate pH as astimulus, at constant ionic strength, using pH-insensitive fluorophores coupled within the brush (donors) and on-chain ends (acceptors), leading to conformational FRET. The influence of pH on the FRET brushes is proved by ellipsometry and fluorescence spectroscopy. Importantly, using FRET meant chain conformation is spatially resolved with sub-micrometer resolution by confocal laser scanning microscopy, where subtle changes in brush conformation are resolved in seconds. Unique mixing dynamics of different pH microdroplets on the brushes are identified as coalescence occurred, with reversible output, and a clear delay in brush responses to mixing liquids. The surfaces offer a new basis for probing conformational transitions of PEBs with high spatiotemporal resolution.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.