Evidence map›Paper›PMID 39836298›Full record

ArticleCurrent microbiology2025

Thymol as a Novel Plant-Derived Antibacterial Agent for Suppressing Xanthomonas citri pv. malvacearum in Cotton.

Muhammad Naveed Aslam, Huma Khaliq, Hongwei Zhao, Anam Moosa, Ambreen Maqsood, Muhammad Aslam Farooqi, Muhammad Saqib Bilal, Tahir Mahmood, Tariq Mukhtar

Abstract read
PubMed Publisher
In one paragraph

Article in Current microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Chemical Profile and Antibacterial Effect ofPlants (Basel, Switzerland) · 2026
    Article
  3. Review
  4. Botanical-Based Strategies for ControllingPlants (Basel, Switzerland) · 2025
    Article
  5. The nematicidal potential ofFrontiers in plant science · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Muhammad Naveed AslamDepartment of Plant Pathology, Faculty of Agriculture and Environment, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.
Huma KhaliqDepartment of Plant Pathology, Faculty of Agriculture and Environment, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.
Hongwei ZhaoDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, China.
Anam MoosaDepartment of Plant Pathology, Faculty of Agriculture and Environment, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.
Ambreen MaqsoodDepartment of Plant Pathology, Faculty of Agriculture and Environment, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.
Muhammad Aslam FarooqiDepartment of Entomology, Faculty of Agriculture and Environment, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.
Muhammad Saqib BilalState Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, China.
Tahir MahmoodDepartment of Plant Pathology, Faculty of Agriculture and Environment, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.
Tariq MukhtarDepartment of Plant Pathology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan. drtmukhtar@uaar.edu.pk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Xanthomonas citri pv. malvacearum (Xcm) associated with bacterial blight disease is a significant and widespread pathogen affecting cotton worldwide. The excessive use of harmful chemicals to control plant pathogens has exerted a negative impact on environmental safety. Thymol is a monoterpene phenol present in the essential oils of plants belonging to Lamiaceae family. In this study the antimicrobial activity of thymol was evaluated against Xcm. The minimum inhibitory concentration (MIC) and 99.9% bactericidal concentration (MBC) of thymol against Xcm were 2 and 4 mg/mL, respectively. The effect of MIC and MBC of thymol against Xcm was assessed on the Luria-Bertani medium. The effect of thymol on intercellular ATP levels, membrane potential, and motility in Xcm was assessed using fluorescence spectrometry for membrane potential and firefly luciferase-based assay for ATP levels. Thymol ruptured the cellular membrane of Xcm, resulting in decreased intracellular ATP concentrations, intracellular leakage of genetic material, and changes in membrane potential. Scanning electron microscopy images supported the impact of thymol on the cell membrane of Xcm. Moreover, thymol inhibited the swimming motility and biofilm formation of Xcm at concentrations equal to or above the MIC and MBC. In contrast, sub-MIC concentrations of thymol had little to no impact on the virulence of Xcm. In conclusion, thymol demonstrated the potential as a strong bactericidal compound against Xcm.

Indexed as

Anti-Bacterial AgentsGossypiumThymolXanthomonasAdenosine TriphosphateBiofilmsCell MembraneMembrane PotentialsMicrobial Sensitivity TestsPlant DiseasesAdenosine TriphosphateAnti-Bacterial AgentsThymolBiofilm formationBlightDisease: PhytochemicalInhibitionPathogenicity

Identifiers

PMID39836298

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.