Evidence map›Paper›PMID 39836216›Full record

ArticleApplied microbiology and biotechnology2025

The PurR family transcriptional regulator promotes butenyl-spinosyn production in Saccharopolyspora pogona.

Xinying Li, Jingnan Wang, Chang Su, Chao Guo, Zhouqin Xu, Kehui Wang, Jian Pang, Bo Lv, Chao Wang, Chun Li

Erratum issuedAbstract read
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In one paragraph

Article in Applied microbiology and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Xinying LiKey Laboratory of Medical Molecule Science and Pharmaceutics Engineering, Ministry of Industry and Information Technology, Institute of Biochemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing, 100081, China.
Jingnan WangAcademy of National Food and Strategic Reserves Administration, Grain and Oils Processing Research Institute, Beijing, 100037, China.
Chang SuAcademy of National Food and Strategic Reserves Administration, Grain and Oils Processing Research Institute, Beijing, 100037, China.
Chao GuoAcademy of National Food and Strategic Reserves Administration, Grain and Oils Processing Research Institute, Beijing, 100037, China.
Zhouqin XuAcademy of National Food and Strategic Reserves Administration, Grain and Oils Processing Research Institute, Beijing, 100037, China.
Kehui WangAcademy of National Food and Strategic Reserves Administration, Grain and Oils Processing Research Institute, Beijing, 100037, China.
Jian PangKey Lab for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing, 100084, China.
Bo LvKey Laboratory of Medical Molecule Science and Pharmaceutics Engineering, Ministry of Industry and Information Technology, Institute of Biochemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing, 100081, China. lv-b@bit.edu.cn.
Chao WangAcademy of National Food and Strategic Reserves Administration, Grain and Oils Processing Research Institute, Beijing, 100037, China. wc@ags.ac.cn.
Chun LiKey Laboratory of Medical Molecule Science and Pharmaceutics Engineering, Ministry of Industry and Information Technology, Institute of Biochemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing, 100081, China. lichun@mail.tsinghua.edu.cn.ORCID http://orcid.org/0000-0003-4262-6848

Funding

the Foundation of Key Laboratory of Industrial Biocatalysis. Ministry of Education. Tsinghua University No.2023003the National Key Research and Development Program of China 2023YFA0914700the National Natural Science Foundation of China 22108153
6 · The paper itself

Abstract

Butenyl-spinosyn, derived from Saccharopolyspora pogona, is a broad-spectrum and effective bioinsecticide. However, the regulatory mechanism affecting butenyl-spinosyn synthesis has not been fully elucidated, which hindered the improvement of production. Here, a high-production strain S. pogona H2 was generated by Cobalt-60 γ-ray mutagenesis, which showed a 2.7-fold increase in production compared to the wild-type strain S. pogona ASAGF58. A comparative transcriptomic analysis between S. pogona ASAGF58 and H2 was performed to elucidate the high-production mechanism that more precursors and energy were used to synthesize of butenyl-spinosyn. Fortunately, a PurR family transcriptional regulator TF00350 was discovered. TF00350 overexpression strain RS00350 induced morphological differentiation and butenyl-spinosyn production, ultimately leading to a 5.5-fold increase in butenyl-spinosyn production (141.5 ± 1.03 mg/L). Through transcriptomics analysis, most genes related to purine metabolism pathway were downregulated, and the butenyl-spinosyn biosynthesis gene was upregulated by increasing the concentration of c-di-GMP and decreasing the concentration of c-di-AMP. These results provide valuable insights for further mining key regulators and improving butenyl-spinosyn production. KEY POINTS: • A high production strain of S. pogona H2 was obtained by

Indexed as

Bacterial ProteinsMacrolidesSaccharopolysporaTranscription FactorsGene Expression ProfilingGene Expression Regulation, BacterialMutagenesisBacterial ProteinsMacrolidesTranscription FactorsButenyl-spinosynPurR family transcriptional regulatorSaccharopolyspora pogonaTranscriptomic

Identifiers

PMID39836216
PMCPMC11750948

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.