Evidence map›Paper›PMID 39835128›Full record

ArticleFrontiers in immunology2024

Interleukin-32 positive immune and resident cells in kidney samples from lupus patients: a pilot study.

Simona Truglia, Francesco Ciccia, Silvia Mancuso, Antonella Capozzi, Aroldo Rizzo, Francesca Romana Spinelli, Fulvia Ceccarelli, Tania Colasanti, Cristina Garufi, Francesca Miranda and 3 more

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In one paragraph

Article in Frontiers in immunology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. A single-cell cytokine dictionary of human peripheral blood.bioRxiv : the preprint server for biology · 2025
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Simona TrugliaRheumatology Unit, Department of Clinical Internal, Anesthesiologic and Cardiovascular Sciences, "Sapienza" University of Rome, Rome, Italy.
Francesco CicciaDepartment of Precision Medicine, University of Campania Luigi Vanvitelli, Naples, Italy.
Silvia MancusoRheumatology Unit, Department of Clinical Internal, Anesthesiologic and Cardiovascular Sciences, "Sapienza" University of Rome, Rome, Italy.
Antonella CapozziDepartment of Experimental Medicine, "Sapienza" University of Rome, Rome, Italy.
Aroldo RizzoPathology Section, Azienda Ospedaliera Ospedali Riuniti Villa Sofia Cervello, Palermo, Italy.
Francesca Romana SpinelliRheumatology Unit, Department of Clinical Internal, Anesthesiologic and Cardiovascular Sciences, "Sapienza" University of Rome, Rome, Italy.
Fulvia CeccarelliRheumatology Unit, Department of Clinical Internal, Anesthesiologic and Cardiovascular Sciences, "Sapienza" University of Rome, Rome, Italy.
Tania ColasantiRheumatology Unit, Department of Clinical Internal, Anesthesiologic and Cardiovascular Sciences, "Sapienza" University of Rome, Rome, Italy.
Cristina GarufiRheumatology Unit, Department of Clinical Internal, Anesthesiologic and Cardiovascular Sciences, "Sapienza" University of Rome, Rome, Italy.
Francesca MirandaRheumatology Unit, Azienda Sanitaria Locale (ASL) Roma1, Rome, Italy.
Maurizio SoriceDepartment of Experimental Medicine, "Sapienza" University of Rome, Rome, Italy.
Cristiano AlessandriRheumatology Unit, Department of Clinical Internal, Anesthesiologic and Cardiovascular Sciences, "Sapienza" University of Rome, Rome, Italy.
Fabrizio ContiRheumatology Unit, Department of Clinical Internal, Anesthesiologic and Cardiovascular Sciences, "Sapienza" University of Rome, Rome, Italy.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Lupus nephritis (LN), caused by immune complexes produced Methods: In LN patients, IL-32 was detected in sera samples by ELISA KIT and in kidney tissue by immunohistochemistry. HEK293/T3 cells were incubated with LN-IgG and analyzed for TBK1, phospho-p65 NF-κB and IL-32 by Western blot. Results: We demonstrated IL-32 presence in LN patients compared to SLE patients without renal involvement, observing a direct correlation between IL-32 serum levels and disease duration (p=0.02; r 0.2978). Moreover, IL-32 was strongly expressed in renal samples of LN patients. Phosphorylation of TBK1 resulting in NF-κB activation and IL-32 increase was observed in HEK293/T3 cells following LN-IgG treatment, TLR3 inhibitor using induced a significant reduction in the expression of these molecules. Discussion: These results showed that IL-32 is up-regulated in the kidney of LN patients suggesting that in renal tissue IL-32 expression could be induced through TLR3 activation by the LN patients' antibodies. This study may indicate a possible role for IL-32 in the pathogenesis of LN.

Indexed as

InterleukinsKidneyLupus Erythematosus, SystemicLupus NephritisAdultFemaleHEK293 CellsHumansImmunoglobulin GMaleMiddle AgedPilot ProjectsProtein Serine-Threonine KinasesToll-Like Receptor 3Young AdultIL32 protein, humanImmunoglobulin GInterleukinsProtein Serine-Threonine KinasesTLR3 protein, humanToll-Like Receptor 3interleukin-32lupus nephritislupus nephritis IgGresident renal cellstoll like receptor 3

Identifiers

PMID39835128
PMCPMC11743457

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