Evidence map›Paper›PMID 39831626›Full record

ArticleRNA biology2025

RNase P cleavage of pseudoknot substrates reveals differences in active site architecture that depend on residue N-1 in the 5' leader.

David M Kosek, J Luis Leal, Ema Kikovska-Stojanovska, Guanzhong Mao, Shiying Wu, Samuel C Flores, Leif A Kirsebom

Abstract read
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In one paragraph

Article in RNA biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

David M KosekDepartment of Cell and Molecular Biology, Biomedical Centre, Uppsala University, Uppsala, Sweden.
J Luis LealDepartment of Cell and Molecular Biology, Biomedical Centre, Uppsala University, Uppsala, Sweden.
Ema Kikovska-StojanovskaDepartment of Cell and Molecular Biology, Biomedical Centre, Uppsala University, Uppsala, Sweden.
Guanzhong MaoDepartment of Cell and Molecular Biology, Biomedical Centre, Uppsala University, Uppsala, Sweden.
Shiying WuDepartment of Cell and Molecular Biology, Biomedical Centre, Uppsala University, Uppsala, Sweden.
Samuel C FloresDepartment of Cell and Molecular Biology, Biomedical Centre, Uppsala University, Uppsala, Sweden.
Leif A KirsebomDepartment of Cell and Molecular Biology, Biomedical Centre, Uppsala University, Uppsala, Sweden.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We show that a small biotin-binding RNA aptamer that folds into a pseudoknot structure acts as a substrate for bacterial RNase P RNA (RPR) with and without the RNase P C5 protein. Cleavage in the single-stranded region in loop 1 was shown to depend on the presence of a RCCA-motif at the 3' end of the substrate. The nucleobase and the 2'hydroxyl at the position immediately 5' of the cleavage site contribute to both cleavage efficiency and site selection, where C at this position induces significant cleavage at an alternative site, one base upstream of the main cleavage site. The frequencies of cleavage at these two sites and Mg

Indexed as

5' Untranslated RegionsRibonuclease PAptamers, NucleotideBase SequenceBinding SitesCatalytic DomainMagnesiumModels, MolecularNucleic Acid ConformationProtein BindingSubstrate Specificity5' Untranslated RegionsAptamers, NucleotideMagnesiumRibonuclease Pdivalent metal ionsmodel substratesribozymeRNase PtRNA processing

Identifiers

PMID39831626
PMCPMC12710915

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.