ArticleRSC advances2025
Rapid detection of hydrogen peroxide and nitrite in adulterated cow milk using enzymatic and nonenzymatic methods on a reusable platform.
Article in RSC advances, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
1 citing paper in PubMed.
- Smartphone-based colorimetric detection of milk adulterationRSC advances · 2025Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cow milk is readily adulterated due to its complex properties that can emulsify many adulterants. Among the commonly used adulterants in cow milk are hydrogen peroxide (HP) and nitrite. Commercially available HP is added to extend cow milk's shelf life, while nitrite enters through the tap or pond water added to increase cow milk's volume. HP disrupts human free radical balance, while nitrite can cause methemoglobinemia. This study aims to rapidly detect these adulterants on-site by developing a point-of-care kit. A modified streptavidin-horseradish peroxidase (Strep-HRP)-tetramethylbenzidine (TMB) assay was used for designing the biosensor for HP detection. The Strep-HRP complex was immobilized on 8-well polystyrene strips with glutaraldehyde crosslinking. TMB was used as the substrate to detect HP at concentrations of 0.04% (v/v) and higher. Nitrite was detected using a modified Griess assay, wherein for the biosensor, the Griess reagent was coated on polystyrene strips with polyethylene glycol (PEG) used as the stabilizer to identify nitrite concentrations of 32 μg mL
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Registered trials
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