ArticleACS omega2025
Selected Ion Extraction of Peptides with Heavy Isotopes and Hydrogen Loss Reduces the Type II Error in Plasma Proteomics.
Article in ACS omega, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Authors and funding
7 authors.
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Abstract
Naturally occurring peptides display a wide mass distribution after ionization due to the presence of heavy isotopes of C, H, N, O, and S and hydrogen loss. There is a crucial need for sensitive methods that collect as much information as possible about all plasma peptide forms. Statistical analysis of the delta mass distribution of peptide precursors from MS/MS spectra that were matched to 63,077 peptide sequences by X!TANDEM revealed Gaussian peaks representing heavy isotopes and hydrogen loss at integer delta mass values of -3, -2, -1, 0, +1, +2, +3, +4, and +5 Da. Human plasma samples were precipitated in acetonitrile, and the resulting proteins were collected over a quaternary amine resin, eluted with NaCl, digested with trypsin, and analyzed by nano liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) with an orbital ion trap (OIT). Fragment spectra (MS/MS) generated from the OIT data were fit to human fully tryptic peptides by X!TANDEM, which led to the identification of 3,888 protein gene symbols represented by three or more peptides (
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