Evidence map›Paper›PMID 39828694›Full record

ArticleScientific reports2025

A combined recombinase polymerase amplification CRISPR/Cas12a assay for detection of Fusarium oxysporum f. sp. cubense tropical race 4.

Megan Ceris Matthews, Jos van der Linden, Isabelle Robène, Samuel Rozsasi, Beatrix Coetzee, Manuela Campa, Johan Burger, Uzoma Nobel Akwuruoha, Ndubuisi Johnkennedy Madufor, Willem Perold and 3 more

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In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Megan Ceris MatthewsDepartment of Plant Pathology, Faculty of Agrisciences, Stellenbosch University, Matieland, 7602, South Africa.
Jos van der LindenDepartment of Plant Pathology, Faculty of Agrisciences, Stellenbosch University, Matieland, 7602, South Africa.
Isabelle RobèneDepartment of Plant Pathology, Faculty of Agrisciences, Stellenbosch University, Matieland, 7602, South Africa.
Samuel RozsasiDepartment of Plant Pathology, Faculty of Agrisciences, Stellenbosch University, Matieland, 7602, South Africa.
Beatrix CoetzeeDepartment of Plant Pathology, Faculty of Agrisciences, Stellenbosch University, Matieland, 7602, South Africa.
Manuela CampaDepartment of Genetics, Faculty of Agrisciences, Stellenbosch University, Matieland, 7602, South Africa.
Johan BurgerDepartment of Genetics, Faculty of Agrisciences, Stellenbosch University, Matieland, 7602, South Africa.
Uzoma Nobel AkwuruohaDepartment of Electrical and Electronic Engineering, Faculty of Engineering, Stellenbosch University, Matieland, 7602, South Africa.
Ndubuisi Johnkennedy MaduforDepartment of Electrical and Electronic Engineering, Faculty of Engineering, Stellenbosch University, Matieland, 7602, South Africa.
Willem PeroldDepartment of Electrical and Electronic Engineering, Faculty of Engineering, Stellenbosch University, Matieland, 7602, South Africa.
Umezuruike Linus OparaSARChI Postharvest Technology Laboratory, Africa Institute for Postharvest Technology, Faculty of AgriSciences, Stellenbosch University, Matieland, 7602, South Africa.
Altus ViljoenDepartment of Plant Pathology, Faculty of Agrisciences, Stellenbosch University, Matieland, 7602, South Africa.
Diane MostertDepartment of Plant Pathology, Faculty of Agrisciences, Stellenbosch University, Matieland, 7602, South Africa. diane@sun.ac.za.

Funding

Horizon 2020 Framework Programme 890856National Research Foundation 138109
6 · The paper itself

Abstract

The soilborne pathogen Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4) is currently devastating banana production worldwide. Once introduced, it is not possible to eradicate the pathogen from soils where it can survive for decades. The only management option available then is to replace Foc TR4-susceptible with -resistant varieties. Timely detection of the pathogen, however, is an important strategy to prevent the introduction of Foc TR4 into new areas and prevent its spread from infested sites. In this study, a single-tube detection technique was developed by combining recombinase polymerase amplification (RPA) and clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a technology (RPA-Cas12a) for detection of Foc TR4. The RPA-Cas12a assay was conducted isothermally, had a sensitivity of up to 10 fg target DNA and did not cross react with any of the 76 non-target isolates included in the specificity testing. The RPA-Cas12a assay detected Foc TR4 from naturally infected banana samples collected in the field and visualization was possible with the naked eye under LED blue light transillumination. The method can be integrated with inexpensive fluorescent or electronic detection devices to accelerate Foc TR4 in-field detection and, thereby, fast-track disease containment strategies.

Indexed as

CRISPR-Cas SystemsFusariumNucleic Acid Amplification TechniquesPlant DiseasesRecombinasesBacterial ProteinsCRISPR-Associated ProteinsEndodeoxyribonucleasesMusaSensitivity and SpecificityBacterial ProteinsCas12a proteinCRISPR-Associated ProteinsEndodeoxyribonucleasesRecombinasesBanana Fusarium wiltCRISPR/Cas12aDisease diagnosisFusarium oxysporum f. sp. cubense tropical race 4Rapid detection

Identifiers

PMID39828694
PMCPMC11743600

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.