ArticleInternational journal of fertility & sterility2025
Impact of Varicocele on The Expression of Testicular Enzymes Involved in The Transsulfuration Pathway.
Article in International journal of fertility & sterility, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOxidative aggression is a hallmark of varicocele and may depend on decreased reactive ability of the endogenous antioxidant system following heat stress. We aimed to investigate the underlying mechanisms. Therefore, the expression of the main enzyme proteins involved in the generation of endogenous antioxidant power, cystathionine beta-synthase (CBS), cystathionine gamma-lyase (CSE), heme oxygenase (HO-1), and also, some of the metabolites (methionine, homocysteine, taurine and vitamin B6) reporting on their activity was investigated using a surgical varicocele model in rats. MATERIALS AND
methodsIn this experimental study, thirty male Wistar rats (7-8 weeks old, weighing 180-220 g) were divided into three groups: control, sham, and varicocele. We evaluated sperm parameters and functional tests, as well as the expression of CBS, CSE, and HO-1 proteins using Western blot analysis. Serum levels of methionine, homocysteine, and taurine were analyzed through high-performance liquid chromatography, while vitamin B6 concentrations were measured using enzyme-linked immunosorbent assay. For group comparisons, we employed ANOVA and the Tukey test, considering a P<0.05 as significant.
resultsWe observed a significant reduction in both sperm quality and functional parameters. Additionally, there was a notable decrease in the expression of CBS, CSE, and HO-1 proteins, as well as circulating vitamin B6 levels, in the varicocele group compared to the control and sham groups (P<0.001).
conclusionThe results of this study suggest that one possible cause of increased oxidative stress in varicocele may be the reduced expression of testicular enzymes involved in the production of endogenous antioxidants, which are associated with the transsulfuration pathway. However, further studies are needed to confirm these findings.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.