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ArticleMolecular biology reports2025

Range-wide understanding of genetic diversity and population structure of endangered Kashmir musk deer in North-Western Himalaya.

Amira Sharief, Vinaya Kumar Singh, Vineet Kumar, Hemant Singh, Avijit Ghosh, Bheem Dutt Joshi, Chinnasamy Ramesh, Mukesh Thakur, Lalit Kumar Sharma

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Article in Molecular biology reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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9 authors.

Amira ShariefZoological Survey of India, Kolkata, 700053, India.
Vinaya Kumar SinghZoological Survey of India, Kolkata, 700053, India.
Vineet KumarZoological Survey of India, Kolkata, 700053, India.
Hemant SinghZoological Survey of India, Kolkata, 700053, India.
Avijit GhoshZoological Survey of India, Kolkata, 700053, India.
Bheem Dutt JoshiZoological Survey of India, Kolkata, 700053, India.
Chinnasamy RameshPopulation Management, Capture & Rehabilitation, Wildlife Institute of India, Dehradun, Uttarakhand, 248001, India.
Mukesh ThakurZoological Survey of India, Kolkata, 700053, India.
Lalit Kumar SharmaZoological Survey of India, Kolkata, 700053, India. lalitganga@gmail.com.

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6 · The paper itself

Abstract

backgroundThe endangered Kashmir musk deer (Moschus cupreus), native to high-altitude Himalayas, is an ecological significant and endangered ungulate, threatened by habitat loss and poaching for musk pod distributed in western Himalayan ranges of India, Nepal and Afghanistan. Despite its critical conservation status and ecological importance in regulating vegetation dynamics, knowledge gaps persist regarding its population structure and genetic diversity, hindering effective management strategies. METHODS AND

resultsWe aimed to understand the population genetics of Kashmir musk deer in north-western Himalayas using two mitochondrial DNA (mtDNA) regions and 11 microsatellite loci. Total of 63 consensus sequences were analyzed, revealing 10 haplotypes with high haplotype diversity (Hd = 0.906 ± 0.031) and moderate nucleotide diversity (π = 0.00539 ± 0.004) at mitochondrial markers. Microsatellite analysis indicated moderate genetic variability, with observed heterozygosity (Ho = 0.445 ± 0.049) and expected heterozygosity (He = 0.764 ± 0.039), comparable to the forest musk deer but relatively lower than that observed in other ungulates. Population structure analysis identified three potential genetic clusters, with evidence of genetic admixture in the Lahaul Valley, possibly due to gene flow from neighboring areas. Historical demographic analyses using Bayesian skyline plots and mismatch distribution curves indicated stable population sizes in the past, followed by recent declines in maternal effective population size.

conclusionsOur findings highlight the necessity of preserving the genetic diversity of the Kashmir musk deer to ensure its long-term survival. We recommend expanding the geographic range of sampling to include regions such as Chamba and Kinnaur districts in Himachal Pradesh, potential areas in Jammu and Kashmir and Uttarakhand, for more comprehensive population-level analysis. Such efforts will be crucial for developing targeted conservation strategies and mitigating the impacts of human activities on this endangered species.

Indexed as

DeerGenetic VariationAnimalsBayes TheoremConservation of Natural ResourcesDNA, MitochondrialEndangered SpeciesGene FlowGenetics, PopulationHaplotypesIndiaMicrosatellite RepeatsPhylogenyDNA, MitochondrialDemographyGenetic diversityKashmir musk deerPopulation geneticsWestern himalayas

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