Evidence map›Paper›PMID 39825415›Full record

ArticleJournal of nanobiotechnology2025

Biofunctionalized patterned platform as microarray biochip to supervise delivery and expression of pDNA nanolipoplexes in stem cells via mechanotransduction.

Mingkui Shen, Yan Hou, Shihui Xu, Jun Tan, Honggang Zhou, Qi Miao, Wanheng Zhang, Yazhou Chen, Nana Wang, Yongtao Wang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of nanobiotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Mingkui ShenDepartment of Mini-Invasive Spinal Surgery, The Third People's Hospital of Henan Province, Zhengzhou, 450000, China.
Yan HouSchool of Medicine, Shanghai University, Shanghai, 200444, China.
Shihui XuSchool of Medicine, Shanghai University, Shanghai, 200444, China.
Jun TanDepartment of Mini-Invasive Spinal Surgery, The Third People's Hospital of Henan Province, Zhengzhou, 450000, China.
Honggang ZhouDepartment of Mini-Invasive Spinal Surgery, The Third People's Hospital of Henan Province, Zhengzhou, 450000, China.
Qi MiaoDepartment of Pharmacy, China Pharmaceutical University, Nanjing, 210009, China.
Wanheng ZhangDepartment of Pharmacy, China Pharmaceutical University, Nanjing, 210009, China.
Yazhou ChenMedical 3D Printing Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, 450052, China. yzchenbio@zzu.edu.cn.
Nana WangDepartment of Pediatrics, School of Medicine, Shanghai General Hospital, Shanghai Jiao Tong University, Shanghai, 200080, China. wang-nana@sjtu.edu.cn.
Yongtao WangSchool of Medicine, Shanghai University, Shanghai, 200444, China. yongtao_wang@shu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Biochips are widely applied to manipulate the geometrical morphology of stem cells in recent years. Patterned antenna-like pseudopodia are also probed to explore the influence of pseudopodia formation on gene delivery and expression on biochips. However, how the antenna-like pseudopodia affect gene transfection is unsettled and the underlying trafficking mechanism of exogenous genes in engineered single cells is not announced. Therefore, the engineered microarray biochips were conceptualized and prepared by the synthesized photointelligent biopolymer to precisely manage geometric topological structures (cell size and antenna-like protrusion) of stem cells on biochips. The cytoskeleton could be regulated in engineered cells and large cells with more antennas assembled well-organized actin filaments to affect cell tension distribution. The stiffness and adhesion force were measured by atomic force microscope to reveal cell nanomechanics on microarray biochips. Cytoskeleton-mediated nanomechanics could be adjusted by actin filaments. Gene transfection efficiency was enhanced with increasing cell nanomechanics, which was also confirmed by the evaluation of cell internalization capacity of nanoparticles and DNA synthesis ability. This work will provide a new strategy to study functional biomaterials, microarray chips and internal mechanism of gene transfection in patterned stem cells on biochips.

Indexed as

DNALiposomesMechanotransduction, CellularNanoparticlesPlasmidsStem CellsActin CytoskeletonAnimalsGene Transfer TechniquesHumansMiceMicroscopy, Atomic ForceTransfectionDNALiposomesCytoskeletal nanomechanicsGene delivery and expressionIntelligent microarray biochipsMechanotransductionPatterned stem cells

Identifiers

PMID39825415
PMCPMC11748598

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.