Exploration of linear and interpretable models for quantification of cell parameters via contactless short-wave infrared hyperspectral sensing.

The development of optical sensors for label-free quantification of cell parameters has numerous uses in the biomedical arena. However, using current optical probes requires the laborious collection of sufficiently large datasets that can be used to calibrate optical probe signals to true metabolite concentrations. Further, most practitioners find it difficult to confidently adapt black box chemometric models that are difficult to troubleshoot in high-stakes applications such as biopharmaceutical manufacturing. Replacing optical probes with contactless short-wave infrared (SWIR) hyperspectral cameras allows efficient collection of thousands of absorption signals in a handful of images. This high repetition allows for effective denoising of each spectrum, so interpretable linear models can quantify metabolites. To illustrate, an interpretable linear model called L-SLR is trained using small datasets obtained with a SWIR HSI camera to quantify fructose, viable cell density (VCD), glucose, and lactate. The performance of this model is also compared to other existing linear models, namely Partial Least Squares (PLS) and Non-negative Matrix Factorization (NMF). Using only 50% of the dataset for training, reasonable test performance of mean absolute error (MAE) and correlations (r