ArticleScience China. Life sciences2025
Reversing anther thermotolerance by manipulating the cis-elements in the promoter of a high-temperature upregulated gene Casein Kinase I in upland cotton.
Article in Science China. Life sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed.
- A structural variation-driven evolutionary history of upland cotton.Science China. Life sciences · 2026Article
- Integrating molecular networks and physiological adaptation for heat-resilient crops.Journal of integrative plant biology · 2026Review
- Designed Alleles of ZmRap2.7 Decouple the Trade-Off Between Early Flowering and Yield Penalty.Plant biotechnology journal · 2026Article
- Robust CRISPR/Cas12a genome editing in Brassica vegetables.Science China. Life sciences · 2026Article
- Genome-Wide Identification of theInternational journal of molecular sciences · 2026Article
- Molecular mechanisms and breeding strategies for heat tolerance in vegetable crops under global warming.Horticulture research · 2026Article
- Comprehensive genome-wide identification and analysis of MYB transcription factors related to abiotic and biotic stress regulation in rice.Scientific reports · 2025Article
- Rational design of promoter editing confers multipathogen resistance in rice.Genome biology · 2025Article
- CRISPR/Cas-Mediated Optimization of Soybean Shoot Architecture for Enhanced Yield.International journal of molecular sciences · 2025Review
- Genome-Wide Characterization of theGenes · 2025Article
- Cotton under heat stress: a comprehensive review of molecular breeding, genomics, and multi-omics strategies.Frontiers in genetics · 2025Review
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8 authors.
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Abstract
High temperature (HT) stress causes male sterility, leading to reduced upland cotton yield. Previously, we identified a key gene, Casein Kinase I (GhCKI), that negatively regulates male fertility in upland cotton under HT. However, conventional genetic manipulations of GhCKI would result in male sterility, hindering its utilization in breeding programs. Here, we engineered quantitative variation for anther thermotolerance-related traits in upland cotton by creating weak promoter alleles of GhCKI genes, using CRISPR/Cas9 and CRISPR/Cpf1 genome editing. Then, we screened and identified two new upland cotton plant lines exhibiting a HT-tolerant phenotype with edited GhCKI promoters, and characterized their corresponding heat-tolerant allelic genotypes. Further research revealed that the primary reason for the HT tolerance of the GhCKI promoter editing mutants is that the trans-acting factors GhMYB73 and GhMYB4, which positively regulate GhCKI expression under HT, failed to bind and activate the expression of GhCKI. Overall, our study not only provides a rapid strategy to generate new beneficial alleles but also offers novel germplasm resources and molecular insights for crop HT tolerance breeding.
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