Evidence map›Paper›PMID 39821735›Full record

ArticlePlant molecular biology2025

Microsporocytic ARF17 misexpression leads to an excess callose deposition and male sterility in Arabidopsis.

Wenxin Su, Jing Huang, Bo Wang, Yaqi Liu, Yijia Chen, Yingyin Li, Naiying Yang, Kaiqi Wang, Xiaofeng Xu

Abstract read
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In one paragraph

Article in Plant molecular biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Wenxin Su *Shanghai Collaborative Innovation Center of Plant Germplasm Resources Development, College of Life Sciences, Shanghai Normal University, Shanghai, China.
Jing Huang *Shanghai Collaborative Innovation Center of Plant Germplasm Resources Development, College of Life Sciences, Shanghai Normal University, Shanghai, China.
Bo WangShanghai Collaborative Innovation Center of Plant Germplasm Resources Development, College of Life Sciences, Shanghai Normal University, Shanghai, China.
Yaqi LiuShanghai Collaborative Innovation Center of Plant Germplasm Resources Development, College of Life Sciences, Shanghai Normal University, Shanghai, China.
Yijia ChenShanghai Collaborative Innovation Center of Plant Germplasm Resources Development, College of Life Sciences, Shanghai Normal University, Shanghai, China.
Yingyin LiShanghai Collaborative Innovation Center of Plant Germplasm Resources Development, College of Life Sciences, Shanghai Normal University, Shanghai, China.
Naiying YangShanghai Collaborative Innovation Center of Plant Germplasm Resources Development, College of Life Sciences, Shanghai Normal University, Shanghai, China. yangnaiying@shnu.edu.cn.
Kaiqi WangCollege of Biological and Environmental Engineering, Jingdezhen University, Jiangxi, China. wang_kaiqi@jdzu.edu.cn.ORCID http://orcid.org/0000-0001-6589-3194
Xiaofeng XuShanghai Collaborative Innovation Center of Plant Germplasm Resources Development, College of Life Sciences, Shanghai Normal University, Shanghai, China. xiaofengxu@shnu.edu.cn.ORCID http://orcid.org/0000-0002-5718-8927

Funding

National Natural Science Foundation of China 32470345Science and Technology Office of Jingdezhen 20224SF007-1Shanghai Engineering Research Center of Plant Germplasm Resources 17DZ2252700
6 · The paper itself

Abstract

The accurate callose deposition plays important roles in pollen wall formation and pollen fertility. As a direct target of miRNA160, ARF17 participate in the formation of the callose wall. However, the impact of ARF17 misexpression in microsporocytes on callose wall formation and pollen fertility remains unknown. Here, the SDS promoter, which is capable of specifically driving gene expression in microsporocytes, was employed to drive the expression of 5mARF17. The pSDS:5mARF17#3 transgenic line were male sterile. TEM revealed that sporopollenin substance was embedded in a thicker callose layer, which resulted in the complete loss of exine structure and pollen abortion in the pSDS:5mARF17#3 line. Consistently, RT-qPCR revealed an increase in the expression of several Cals genes in pSDS:5mARF17#3. EMSA assay demonstrated that ARF17 could bind to the promoter of Cals4 gene, which further suggest that ARF17 could regulate several Cals genes expression. It is notable that the expression of several exine formation-related genes increased significantly in pSDS:5mARF17#3. In conclusion, our findings highlight that the regulation of miRNA160-ARF17 in microsporocytes modulates the thickness of the callose wall, which is crucial for pollen exine formation and intercellular communication.

Indexed as

ArabidopsisArabidopsis ProteinsGlucansPlant InfertilityPollenTranscription FactorsCell WallGene Expression Regulation, PlantMicroRNAsPlants, Genetically ModifiedPromoter Regions, GeneticArabidopsis ProteinscalloseGlucansMicroRNAsTranscription FactorsARF17Callose wallMale sterilitymiRNA160Pollen wall

Identifiers

PMID39821735

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.