Evidence map›Paper›PMID 39821568›Full record

ArticleClinical and experimental nephrology2025

Evaluation of renal fibrosis using scanning acoustic microscopy.

Takane Ito, Hideki Kumagai, Takahiro Kanai, Jun Aoyagi, Yuko Ono, Katsutoshi Miura, Kazuto Kobayashi, Toshihiro Tajima, Hitoshi Osaka

Abstract readComparative Study
PubMed Publisher
In one paragraph

Article in Clinical and experimental nephrology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Takane ItoDepartment of Pediatrics, Jichi Medical University, Tochigi, Japan.
Hideki KumagaiDepartment of Pediatrics, Jichi Medical University, Tochigi, Japan. h-kumagai@jichi.ac.jp.ORCID http://orcid.org/0000-0001-5309-8396
Takahiro KanaiDepartment of Pediatrics, Jichi Medical University, Tochigi, Japan.
Jun AoyagiDepartment of Pediatrics, Jichi Medical University, Tochigi, Japan.
Yuko OnoDivision of Nephrology, Department of Internal Medicine, Jichi Medical University, Tochigi, Japan.
Katsutoshi MiuraDepartment of Regenerative and Infectious Pathology, Hamamatsu University School of Medicine, Hamamatsu, Japan.
Kazuto KobayashiHonda Electronics Co., Ltd., Toyohashi, Japan.
Toshihiro TajimaDepartment of Pediatrics, Jichi Medical University, Tochigi, Japan.
Hitoshi OsakaDepartment of Pediatrics, Jichi Medical University, Tochigi, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundRenal fibrosis is strongly correlated with renal functional outcomes. Therefore, this is a significant finding in determining renal prognosis. There are various reports on the imaging evaluation of renal fibrosis, but these are not well established. Scanning acoustic microscopy (SAM) uses ultra-high-frequency ultrasound to visualize tissues in just over a minute. SAM can simultaneously measure acoustic data such as speed of sound (SOS). SOS indicates the elasticity (stiffness) of a material. In this study, we aimed to compare and evaluate SAM acoustic intensity images and SOS data with light microscopy images of renal lesions, especially renal fibrosis.

methodsRenal specimens containing fibrosis were selected. The acoustic intensity images were compared to PAS-stained images. SOS data of the tubulointerstitium were compared with Masson's trichrome (MT)-stained images. The blue intensity of MT staining, which indicates fibrosis, was numerically valued using image-processing software. Furthermore, the correlations between it and the SOS values were evaluated.

resultsThe acoustic intensity images suggested tubular atrophy and interstitial expansion in the same areas as in the PAS staining. SOS values of interstitial expansion with fibrosis were higher than normal area, interstitial expansion without fibrosis. A weak positive correlation was observed between the SOS values and the blue intensity of MT staining.

conclusionsSOS data can be used to evaluate renal fibrosis. The combination of SOS data and MT-stained images enables a more detailed evaluation of renal fibrosis. This study can contribute to the evaluation of renal fibrosis and has potential clinical applications in the future.

Indexed as

KidneyKidney DiseasesMicroscopy, AcousticAzo CompoundsEosine Yellowish-(YS)FemaleFibrosisHumansMaleMethyl GreenMiddle AgedAzo CompoundsEosine Yellowish-(YS)Methyl Greentrichrome stainMasson’s trichrome stainingRenal fibrosisScanning acoustic microscopySpeed of soundTubulointerstitiumUltrasound

Identifiers

PMID39821568

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