Evidence map›Paper›PMID 39821095›Full record

ArticleScientific reports2025

Versatile application of fast green FCF as a visible cholangiogram in adult mice to medium-sized mammals.

Tomoyuki Niimi, Nanae Miyazaki, Hironobu Oiki, Mami Uemura, Shihan Zeng, Watcharapon Promsut, Noriaki Ota, Shunji Nonaka, Hajime Takei, Hiroshi Nittono and 7 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Tomoyuki Niimi *Department of Veterinary Anatomy, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113-8657, Japan.
Nanae Miyazaki *Department of Veterinary Anatomy, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113-8657, Japan.
Hironobu Oiki *Department of Veterinary Anatomy, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113-8657, Japan.
Mami UemuraDepartment of Veterinary Anatomy, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113-8657, Japan.
Shihan ZengDepartment of Veterinary Anatomy, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113-8657, Japan.
Watcharapon PromsutDepartment of Veterinary Anatomy, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113-8657, Japan.
Noriaki OtaDepartment of Veterinary Anatomy, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113-8657, Japan.
Shunji NonakaDepartment of Veterinary Anatomy, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113-8657, Japan.
Hajime TakeiJunshin Clinic Bile Acid Institute, Meguro-ku, Tokyo, Japan.
Hiroshi NittonoJunshin Clinic Bile Acid Institute, Meguro-ku, Tokyo, Japan.
Seiko NarushimaRIKEN Center for Integrative Medical Sciences, Yokohama, Kanagawa, Japan.
Ayaka YanagidaDepartment of Veterinary Anatomy, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113-8657, Japan.
Ryuji HiramatsuDepartment of Veterinary Anatomy, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113-8657, Japan.
Masami Kanai-AzumaCenter for Experimental Animals, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan.
Shohei TakamiDepartment of Veterinary Anatomy, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113-8657, Japan.
Jun FujishiroDepartment of Pediatric Surgery, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Yoshiakira KanaiDepartment of Veterinary Anatomy, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113-8657, Japan. ykanai@g.ecc.u-tokyo.ac.jp.

Funding

Japan Agency for Medical Research and Development JP21lm0203003j0005Japan Society for the Promotion of Science 21H00227Japan Society for the Promotion of Science 21H02387
6 · The paper itself

Abstract

An aqueous solution of a common food dye, Fast Green FCF (FG), mimics cholyl-lysyl-fluorescein to visualize embryonic bile flow via single peritoneal injection into intrauterine mouse embryos. Despite its efficacy in embryos, its suitability for adult mice and small to medium-sized mammals remained uncertain. In this study, we investigated FG cholangiography in adult mice, dogs, and goats. The results demonstrate that FG injection enables progressive cholangiography in these species, highlighting its versatility across different animal models without necessitating specialized equipment. To further evaluate diagnostic utility, FG cholangiography was performed in various mouse models of bile flow disorders. FG successfully visualized dilated lumina in the extrahepatic bile duct of BDL mice and revealed aberrant luminal structures in the gallbladder walls of Sox17

Indexed as

CholangiographyFluoresceinsFluorescent DyesAnimalsBileDogsFemaleGallbladderMiceSOXF Transcription FactorsFluoresceinsFluorescent DyesSOXF Transcription FactorsBile flowCholangiographyDogGallbladderGoatMouse

Identifiers

PMID39821095
PMCPMC11739563

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.