Evidence map›Paper›PMID 39819468›Full record

ArticleParasites & vectors2025

Experimental infection of wild boars (Sus scrofa) with Rickettsia rickettsii and evaluation of the transmission potential to Amblyomma sculptum ticks.

Lucianne Cardoso Neves, Lina de Campos Binder, Warley Vieira de Freitas Paula, Nicolas Jalowitzki de Lima, Ennya Rafaella Neves Cardoso, Rayane Almeida Santos, Raphaela Bueno Mendes Bittencourt, Gracielle Teles Pádua, Gabriel Cândido Dos Santos, Mariana Avelar Tavares and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Parasites & vectors, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Lucianne Cardoso NevesVeterinary and Animal Science School, Federal University of Goiás, Goiânia, Goiás, 74690-900, Brazil.
Lina de Campos BinderDepartment of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, 05508-270, Brazil.
Warley Vieira de Freitas PaulaVeterinary and Animal Science School, Federal University of Goiás, Goiânia, Goiás, 74690-900, Brazil.
Nicolas Jalowitzki de LimaVeterinary and Animal Science School, Federal University of Goiás, Goiânia, Goiás, 74690-900, Brazil.
Ennya Rafaella Neves CardosoVeterinary and Animal Science School, Federal University of Goiás, Goiânia, Goiás, 74690-900, Brazil.
Rayane Almeida SantosVeterinary and Animal Science School, Federal University of Goiás, Goiânia, Goiás, 74690-900, Brazil.
Raphaela Bueno Mendes BittencourtVeterinary and Animal Science School, Federal University of Goiás, Goiânia, Goiás, 74690-900, Brazil.
Gracielle Teles PáduaVeterinary and Animal Science School, Federal University of Goiás, Goiânia, Goiás, 74690-900, Brazil.
Gabriel Cândido Dos SantosVeterinary and Animal Science School, Federal University of Goiás, Goiânia, Goiás, 74690-900, Brazil.
Mariana Avelar TavaresVeterinary and Animal Science School, Federal University of Goiás, Goiânia, Goiás, 74690-900, Brazil.
Maria Carolina de Azevedo SerpaDepartment of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, 05508-270, Brazil.
Adriano PinterDepartment of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, 05508-270, Brazil.
Artur Luiz de Almeida FelicioCoordination of Agricultural Defense, Secretary of Agriculture and Supply, Government of the State of São Paulo, São Paulo, Brazil.
Marcelo B LabrunaDepartment of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, 05508-270, Brazil.
Felipe da Silva KrawczakVeterinary and Animal Science School, Federal University of Goiás, Goiânia, Goiás, 74690-900, Brazil. felipekvet@ufg.br.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundBrazilian spotted fever is a tick-borne disease caused by the bacterium Rickettsia rickettsii, whose main vector in Brazil is the tick Amblyomma sculptum. Amplifying hosts are essential for the perpetuation of this bacterium in the tick population as they can be sources of infection during bacteremic periods. Recent studies demonstrated the ability of suids (Sus scrofa) to sustain populations of A. sculptum, one of the main tick species found parasitizing wild boars in the midwestern and southeastern regions of Brazil. In this study, wild boars were experimentally infected with R. rickettsii by tick infestation and were evaluated for their ability to transmit the infection to A. sculptum ticks, under laboratory conditions.

methodsFour wild boars were infected with R. rickettsii through infestation with R. rickettsii-infected A. sculptum adults (infected group); a fifth wild boar was infested with uninfected A. sculptum adults (control group). Simultaneously, the animals were infested with uninfected larvae and nymphs of A. sculptum. The wild boars were monitored for 28 days by clinical examination and hematological tests, real-time quantitative PCR (qPCR) of blood for the detection of Rickettsia and inoculation of blood in guinea pigs. IgG antibody titers were followed until the end of the experiment. Unfed nymphs and adults, molted from engorged larvae and nymphs that fed on wild boars, were used to infest susceptible guinea pigs and rabbits; some of these unfed ticks were tested by qPCR for rickettsial detection.

resultsThe wild boars showed no clinical or hematological alterations, and bacteremia was not detected by qPCR or inoculation of wild boar blood into guinea pigs. Furthermore, wild boars showed a moderate humoral response, with anti-R. rickettsii endpoint titers up to 256 or 512. Rickettsial DNA was not detected in molted ticks after acquisition feeding on wild boars. Moreover, no disease or seroconversion was observed in guinea pigs and rabbits that were infested with ticks originated from wild boar acquisition feeding.

conclusionsWild boars seroconverted to Rickettsia spp. after being infested with R. rickettsii-infected A. sculptum; however, they did not develop bacteremia and did not act as competent amplifying hosts of R. rickettsii for A. sculptum ticks.

Indexed as

AmblyommaArachnid VectorsBacteremiaRickettsia rickettsiiRocky Mountain Spotted FeverSwine DiseasesTick InfestationsAnimalsAnimals, WildBrazilFemaleGuinea PigsLarvaMaleNymphRabbitsAmblyomma sculptumBrazilian spotted feverSuidaeTick-borne diseases

Identifiers

PMID39819468
PMCPMC11740395

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.