Evidence map›Paper›PMID 39817767›Full record

ArticleJournal of bacteriology2025

Screening a library of temperature-sensitive mutants to identify secretion factors in

Owen Leddy, Amany M Ibrahim, Muhammad S Azam, Sadie Solomon, Wenqi Yu, Olaf Schneewind, Dominique Missiakas

Abstract read
In one paragraph

Article in Journal of bacteriology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
  2. Article
  3. Role of staphylococcal EzrA as a molecular organizer of cell division.bioRxiv : the preprint server for biology · 2026
    Article
  4. Article
  5. Article
  6. Article
  7. A novel polysaccharide in the envelope ofJournal of bacteriology · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Owen Leddy *Department of Microbiology, Howard Taylor Ricketts Laboratory, The University of Chicago, Chicago, Illinois, USA.
Amany M Ibrahim *Department of Microbiology, Howard Taylor Ricketts Laboratory, The University of Chicago, Chicago, Illinois, USA.
Muhammad S Azam *Department of Microbiology, Howard Taylor Ricketts Laboratory, The University of Chicago, Chicago, Illinois, USA.
Sadie SolomonDepartment of Microbiology, Howard Taylor Ricketts Laboratory, The University of Chicago, Chicago, Illinois, USA.
Wenqi YuDepartment of Microbiology, Howard Taylor Ricketts Laboratory, The University of Chicago, Chicago, Illinois, USA.ORCID 0000-0001-8772-3129
Olaf SchneewindDepartment of Microbiology, Howard Taylor Ricketts Laboratory, The University of Chicago, Chicago, Illinois, USA.
Dominique MissiakasDepartment of Microbiology, Howard Taylor Ricketts Laboratory, The University of Chicago, Chicago, Illinois, USA.ORCID 0000-0001-6482-9633

Funding

Surface Proteins of Gram-Positive BacteriaR01AI038897 · NIAID · UNIVERSITY OF CHICAGO · PI MISSIAKAS, DOMINIQUE M. · 1996 to 2024
$8.9M
Spatiotemporal regulation of bacterial cell envelope assemblyR35GM146993 · NIGMS · UNIVERSITY OF SOUTH FLORIDA · PI Wenqi Yu · 2022 to 2026
$1.8M
HHS | NIH | National Institute of Allergy and Infectious Diseases (NIAID) AI038897NIAID NIH HHS R01 AI038897NIGMS NIH HHS R35 GM146993
6 · The paper itself

Abstract

Protein secretion is an essential cell process in bacteria, required for cell envelope biogenesis, export of virulence factors, and acquisition of nutrients, among other important functions. In the Sec secretion pathway, signal peptide-bearing precursors are recognized by the SecA ATPase and pushed across the membrane through a translocon channel made of the proteins SecY, SecE, and SecG. The Sec pathway has been extensively studied in the model organism

Indexed as

Bacterial ProteinsMutationStaphylococcus aureusAdenosine TriphosphatasesGene Expression Regulation, BacterialProtein TransportSecA ProteinsSEC Translocation ChannelsTemperatureAdenosine TriphosphatasesBacterial ProteinsSecA ProteinsSEC Translocation ChannelsPepVprotein secretionSecASecGStaphylococcus aureustemperature-sensitive mutant

Identifiers

PMID39817767
PMCPMC11841065

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.