Evidence map›Paper›PMID 39817737›Full record

ArticleApplied and environmental microbiology2025

Comparing field and lab quantitative stable isotope probing for nitrogen assimilation in soil microbes.

Kinsey Reed, Chansotheary Dang, Jeth Walkup, Alicia Purcell, Bruce Hungate, Ember Morrissey

Abstract readComparative Study
In one paragraph

Article in Applied and environmental microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Kinsey ReedDivision of Plant and Soil Sciences, West Virginia University, Morgantown, West Virginia, USA.ORCID 0000-0001-5517-8664
Chansotheary DangDivision of Plant and Soil Sciences, West Virginia University, Morgantown, West Virginia, USA.
Jeth WalkupDivision of Plant and Soil Sciences, West Virginia University, Morgantown, West Virginia, USA.
Alicia PurcellDepartment of Biological Sciences, Texas Tech University, Lubbock, Texas, USA.
Bruce HungateCenter for Ecosystem Science and Society, Northern Arizona University, Flagstaff, Arizona, USA.ORCID 0000-0002-7337-1887
Ember MorrisseyDivision of Plant and Soil Sciences, West Virginia University, Morgantown, West Virginia, USA.ORCID 0000-0002-5810-1096

Funding

U.S. Department of Energy (DOE) DE-SC0020172U.S. Department of Energy (DOE) DE-SC0023126
6 · The paper itself

Abstract

Soil microbial communities play crucial roles in nutrient cycling and can help retain nitrogen in agricultural soils. Quantitative stable isotope probing (qSIP) is a useful method for investigating taxon-specific microbial growth and utilization of specific nutrients, such as nitrogen (N). Typically, qSIP is performed in a highly controlled lab setting, so the field relevance of lab qSIP studies remains unknown. We conducted and compared tandem lab and field qSIP to quantify the assimilation of IMPORTANCE: Soil microbes are responsible for critical biogeochemical processes in natural and agricultural ecosystems. Despite their importance, the functional traits of most soil organisms remain woefully under-characterized, limiting our ability to understand how microbial populations influence the transformation of elements such as nitrogen (N) in soil. Quantitative stable isotope probing (qSIP) is a powerful tool to measure the traits of individual taxa. This method has rarely been applied in the field or with

Indexed as

BacteriaIsotope LabelingNitrogenNitrogen IsotopesSoil MicrobiologyMicrobiotaSoilZea maysNitrogenNitrogen-15Nitrogen IsotopesSoilagroecosystemsmaize microbiomemicrobial communitiesmolecular methodsnitrogen assimilationnitrogen immobilizationquantitative methodsrhizospheresoil microbiology

Identifiers

PMID39817737
PMCPMC11837507

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.