Evidence map›Paper›PMID 39816815›Full record

ArticleFrontiers in parasitology2023

Secreted and surface proteome and transcriptome of

Yide Wong, Mark S Pearson, Olga Fedorova, Vladimir Ivanov, Ekaterina Khmelevskaya, Bemnet Tedla, Buddhika Jayakody Arachchige, Sarah Reed, Matt Field, Thewarach Laha and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in parasitology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Yide WongAustralian Institute of Tropical Health and Medicine, James Cook University, Cairns, QLD, Australia.
Mark S PearsonAustralian Institute of Tropical Health and Medicine, James Cook University, Cairns, QLD, Australia.
Olga FedorovaDepartment of Faculty Pediatrics, Federal State Budget Educational Institution of Higher Education, Siberian State Medical University, Ministry of Healthcare of Russian Federation, Tomsk, Russia.
Vladimir IvanovLaboratory of Biological Models, Federal State Budget Educational Institution of Higher Education, Siberian State Medical University, Ministry of Healthcare of Russian Federation, Tomsk, Russia.
Ekaterina KhmelevskayaCentral Research Laboratory, Federal State Budget Educational Institution of Higher Education, Siberian State Medical University, Ministry of Healthcare of Russian Federation, Tomsk, Tomsk, Russia.
Bemnet TedlaAustralian Institute of Tropical Health and Medicine, James Cook University, Cairns, QLD, Australia.
Buddhika Jayakody ArachchigeMass Spectrometry Facility, Centre for Clinical Research, University of Queensland, Brisbane, QLD, Australia.
Sarah ReedMass Spectrometry Facility, Centre for Clinical Research, University of Queensland, Brisbane, QLD, Australia.
Matt FieldCollege of Public Health, Medical and Veterinary Science, James Cook University, Cairns, QLD, Australia.
Thewarach LahaDepartment of Parasitology, Faculty of Medicine, Khon Kaen Universit, Khon Kaen, Thailand.
Alex LoukasAustralian Institute of Tropical Health and Medicine, James Cook University, Cairns, QLD, Australia.
Javier SotilloAustralian Institute of Tropical Health and Medicine, James Cook University, Cairns, QLD, Australia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Methods: Adult flukes were collected from experimentally infected hamsters and cultured in vitro in serum-free media. We extracted proteins from different compartments of the Results: We also generated a transcriptome using long-read sequencing, and when this was combined with high-resolution mass spectrometry, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) separation, and protein digestion, we identified 686, 894, 389, 324, and 165 proteins from the ES, 15K-EV, and the three sequentially extracted tegument (TEG) protein fractions, respectively. We conducted in-depth gene ontology and protein family analyses on the identified proteins and discussed comparisons against similar proteome data sets acquired for the Southeast Asian liver fluke Discussion: The information from this study will form a biologically relevant data set of

Indexed as

liver flukemicrovesicles (MVs)Opisthorchis felineusproteomicssecretometegument

Identifiers

PMID39816815
PMCPMC11732047

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.