Evidence map›Paper›PMID 39816156›Full record

ArticleBiomedical optics express2025

In vivo three-photon fluorescence imaging of mouse brain vasculature labeled by Evans blue excited at the NIR-III window.

Shen Tong, Hongji Liu, Jie Huang, Jincheng Zhong, Jiemei Yan, Heng Wang, Xiao Zhang, Ping Qiu, Ke Wang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical optics express, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Shen TongSchool of Medical Information and Engineering, Xuzhou Medical University, Xuzhou, Jiangsu, China.
Hongji LiuShenzhen City Polytechnic, Shenzhen, China.
Jie HuangKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen, China.
Jincheng ZhongKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen, China.
Jiemei YanKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen, China.
Heng WangSchool of Medical Information and Engineering, Xuzhou Medical University, Xuzhou, Jiangsu, China.
Xiao ZhangSchool of Medical Information and Engineering, Xuzhou Medical University, Xuzhou, Jiangsu, China.
Ping QiuKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen, China.
Ke WangKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen, China.ORCID https://orcid.org/0000-0002-9445-2168

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Multiphoton fluorescence microscopy (MFM), renowned for its noninvasiveness and high spatiotemporal resolution, is extensively applied in brain structure imaging in vivo. Three-photon fluorescence (3PF) imaging, excited at the NIR-III window, can penetrate the deepest mouse cerebrovascular. Evans blue, a substance known for its low toxicity, high water solubility, and resistance to metabolism, is frequently employed to assess blood-brain barrier (BBB) permeability. However, its suitability for multiphoton fluorescence imaging of mouse cerebrovascular at the NIR-III window in vivo remains unexplored. In this paper, we conduct a comprehensive analysis of the multiphoton excitation and emission characterization of Evans blue when excited at the NIR-III window. Our findings indicate that 1) Evans blue can generate 3PF signals; 2) it exhibits a substantial three-photon action cross-section (

Identifiers

PMID39816156
PMCPMC11729278

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.