Evidence map›Paper›PMID 39816147›Full record

ArticleBiomedical optics express2025

Quantifying UV-induced photodamage for longitudinal live-cell imaging applications of deep-UV microscopy.

Viswanath Gorti, Kaitlyn McCubbins, Daniel Houston, Aaron D Silva Trenkle, Abigail Holberton, Caroline E Serafini, Levi Wood, Gabriel Kwong, Francisco E Robles

Abstract read
In one paragraph

Article in Biomedical optics express, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Rapid, Point-of-Care Bone Marrow Aspirate Adequacy Assessment Via Deep Ultraviolet Microscopy.Laboratory investigation; a journal of technical methods and pathology · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Viswanath GortiWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, USA.ORCID https://orcid.org/0000-0003-0633-5840
Kaitlyn McCubbinsWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, USA.
Daniel HoustonWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, USA.
Aaron D Silva TrenkleWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, USA.
Abigail HolbertonGeorge W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA.
Caroline E SerafiniGeorge W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA.ORCID https://orcid.org/0000-0002-6512-5977
Levi WoodGeorge W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA.
Gabriel KwongWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, USA.
Francisco E RoblesWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, USA.ORCID https://orcid.org/0000-0003-4413-9779

Funding

T32 CTEng (Cellular and Tissue Engineering) Training ProgramT32GM145735 · NIGMS · GEORGIA INSTITUTE OF TECHNOLOGY · PI Edward A. Botchwey, Andres J Garcia · 2022 to 2026
$2.3M
DNA-gated cytometry for multiplexed sorting of antigen-specific CD8 T cellsR01AI171892 · NIAID · GEORGIA INSTITUTE OF TECHNOLOGY · PI KWONG, GABRIEL A · 2022 to 2025
$2.0M
Accessible label-free optical microscopy with quantitative molecular and functional contrastR35GM147437 · NIGMS · ST. JUDE CHILDREN'S RESEARCH HOSPITAL · PI Francisco E Robles · 2022 to 2026
$1.9M
Deep-UV Microscopy for Real-Time Adequacy Analysis of Bone Marrow AspiratesR41EB035057 · NIBIB · CELLIA SCIENCE, INC. · PI ROBLES, FRANCISCO E · 2023 to 2024
$423k
Microfluidic Cartridge for Point-of-Care Hematology AnalysisR43HL167435 · NHLBI · CELLIA SCIENCE, INC. · PI MABRY, KELLY, ROBLES, FRANCISCO E · 2023 to 2023
$276k
NHLBI NIH HHS R43 HL167435NIAID NIH HHS R01 AI171892NIBIB NIH HHS R41 EB035057NIGMS NIH HHS R35 GM147437NIGMS NIH HHS T32 GM145735
6 · The paper itself

Abstract

Deep-UV microscopy enables high-resolution, label-free molecular imaging by leveraging biomolecular absorption properties in the UV spectrum. Recent advances in UV-imaging hardware have renewed interest in this technique for quantitative live cell imaging applications. However, UV-induced photodamage remains a concern for longitudinal dynamic imaging studies. Here, we quantify UV phototoxicity with several cell types at notable UV wavelengths. We find that the fluence required for cell death via UV phototoxicity with continuous UV exposure varies with cell type and wavelength from ∼0.5µJ/µm

Identifiers

PMID39816147
PMCPMC11729288

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.