ArticleJournal of biological engineering2025
Engineering Yarrowia lipolytica for the production of β-carotene by carbon and redox rebalancing.
Article in Journal of biological engineering, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed.
- Coupling Carbon-Source Transport with Antioxidant Biosynthesis in Yeast: Product Diversity and Engineering Strategies.Biology · 2026Review
- Spatial Engineering of Yarrowia lipolytica for the Biosynthesis of Capsanthin.Biotechnology journal · 2026Article
- 10 years of CRISPR/CAS genomic engineering in Yarrowia lipolytica.Bioprocess and biosystems engineering · 2026Review
- Enhanced methanol tolerance and utilization in Pichia pastoris revealed by adaptive laboratory evolution.Journal of biological engineering · 2025Article
- Advances in Lycopene Production: From Natural Sources to Microbial Synthesis UsingMolecules (Basel, Switzerland) · 2025Article
- Advances in the sustainable biosynthesis of valuable terpenoid flavor compounds and precursors in micro-organisms.Biotechnology for biofuels and bioproducts · 2025Review
- Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
backgroundβ-Carotene is a natural product that has garnered significant commercial interest. Considerable efforts have been made to meet such demand through the metabolic engineering of microorganisms, yet there is still potential for improvement. In this study, engineering approaches including carbon and redox rebalancing were used to maximize β-carotene production in Yarrowia lipolytica.
resultsThe initial production level was increased by iterative overexpression of pathway genes with lycopene inhibition removal. For further improvement, two approaches that redirect the central carbon pathway were evaluated to increase NADPH regeneration and reduce ATP expenditure. Pushing flux through the pentose phosphate pathway and introducing NADP
conclusionsThe Y. lipolytica-based β-carotene production chassis was successfully developed through iterative overexpression of multiple pathways, central carbon pathway engineering and lipid pathway flux adjustment. The approach presented here provides insights into future endeavors to improve microbial terpenoid production capability.
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Registered trials
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