ArticleCommunications biology2025
Functional genomics implicates ebony in the black pupae phenotype of tephritid fruit flies.
Article in Communications biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- CRISPR/Cas9-based white pupae mutant lines in Bactrocera spp. for sterile insect technique applications.Insect science · 2026Article
- Neoclassical development of genetic sexing strains for insect pest and disease vector control.Insect science · 2026Review
- Genetic engineering for SIT application: a fruit fly-focused review.Insect science · 2026Review
- Functional validation of a white pupae minimal gene construct in Ceratitis capitata (Diptera: Tephritidae).Insect science · 2026Article
- Development and evaluation of pupal color-based genetic sexing strains in Anastrepha obliqua (Diptera: Tephritidae).Insect science · 2026Article
- Chromosome-scale genome assembly of the South American fruit fly, Anastrepha fraterculus sp.1.Insect science · 2026Article
- Similar Genetic Routes Are Independently Targeted for Mimetic Color Convergence in Bumble Bees.Molecular biology and evolution · 2025Article
- Decoding and engineering temperature-sensitive lethality inProceedings of the National Academy of Sciences of the United States of America · 2025Article
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Authors and funding
18 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The remarkable diversity of insect pigmentation offers a captivating avenue for studying evolution and genetics. In tephritids, understanding the molecular basis of mutant traits is also crucial for applied entomology, enabling the creation of genetic sexing strains through genome editing, thus facilitating sex-sorting before sterile insect releases. Here, we present evidence from classical and modern genetics showing that the black pupae (bp) phenotype in the GUA10 strain of Anastrepha ludens is caused by a large deletion at the ebony locus, removing the gene's entire coding region. Targeted knockout of ebony induced analogous bp phenotypes across six major tephritid agricultural pests, demonstrating that disruption of Ebony alone is sufficient to produce the mutant trait in distantly related species. This functional characterization further allowed a deeper exploration of Ebony's role in pigmentation and development across life stages in diverse species. Our findings offer key insights for molecular engineering of sexing strains based on the bp marker and for future evolutionary developmental biology studies in tephritids.
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