Evidence map›Paper›PMID 39813008›Full record

ArticleAnatomical science international2025

Temporal expression and specific localization of tenomodulin with reference to vascular endothelial growth factor A and calcitonin gene-related peptide in developing diaphragm in mice.

Ryutaro Nakagawa, Iwao Sato, Yoko Ueda, Shinichi Kawata, Yutaro Natsuyama, Tomiko Yakura, Zhong-Lian Li, Hidenobu Miyaso, Masahiro Itoh

Abstract read
PubMed Publisher
In one paragraph

Article in Anatomical science international, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Ryutaro NakagawaDepartment of Anatomy, Tokyo Medical University, 6-1-1, Shinjuku, Shinjuku-ku, Tokyo, 160-8402, Japan.
Iwao SatoDepartment of Anatomy, Tokyo Medical University, 6-1-1, Shinjuku, Shinjuku-ku, Tokyo, 160-8402, Japan. iwaoa1@tokyo-med.ac.jp.ORCID http://orcid.org/0000-0002-7035-2525
Yoko UedaDepartment of Anatomy, Division of Basic Medical Science, Tokai University School of Medicine, 143 Shimokasuya, Isehara-shi, Kanagawa, 259-1193, Japan.
Shinichi KawataDepartment of Anatomy, Tokyo Medical University, 6-1-1, Shinjuku, Shinjuku-ku, Tokyo, 160-8402, Japan.
Yutaro NatsuyamaDepartment of Anatomy, Tokyo Medical University, 6-1-1, Shinjuku, Shinjuku-ku, Tokyo, 160-8402, Japan.
Tomiko YakuraDepartment of Anatomy, Tokyo Medical University, 6-1-1, Shinjuku, Shinjuku-ku, Tokyo, 160-8402, Japan.
Zhong-Lian LiDepartment of Anatomy, Tokyo Medical University, 6-1-1, Shinjuku, Shinjuku-ku, Tokyo, 160-8402, Japan.
Hidenobu MiyasoDepartment of Anatomy, Tokyo Medical University, 6-1-1, Shinjuku, Shinjuku-ku, Tokyo, 160-8402, Japan.
Masahiro ItohDepartment of Anatomy, Tokyo Medical University, 6-1-1, Shinjuku, Shinjuku-ku, Tokyo, 160-8402, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Tenomodulin (TNMD) is related to chondromodulin-1, a cartilage-derived growth regulator. It is specifically expressed in hypovascular connective tissues, including tendons and ligaments. Vascular endothelial growth factor A (VEGF-A) and calcitonin gene-related peptide (CGRP) correlate with angiogenesis and neurogenesis, respectively, during development. Because of that the morphological profiles of TNMD, VEGF-A, and CGRP distributions in developing diaphragm are still unclear, we aimed to determine the expression of mRNA and proteins of these 3 markers during the development of the central tendon of the diaphragm (CTD) and the surrounding diaphragm tissue in mice. Male mice from the embryonic and postnatal periods [embryonic day 14.5 (E14.5), E17.5, postnatal day 1 (P1), and P5] were used and their mRNA and protein expressions were assessed using in situ hybridization, immunohistochemistry and quantitative real-time polymerase chain reaction. The results showed that TNMD was preferentially detected in muscle-tendon junction of the CTD at E17.5 and P1, in contrast to scattered distribution of CGRP and VEGF-A throughout the diaphragm from E14.5 to P5. These findings may provide fundamental information concerning the role of the TNMD in the CTD development.

Indexed as

Calcitonin Gene-Related PeptideDiaphragmMembrane ProteinsVascular Endothelial Growth Factor AAnimalsImmunohistochemistryIn Situ HybridizationMaleMiceRNA, MessengerTendonsCalcitonin Gene-Related PeptideMembrane ProteinsRNA, MessengerVascular Endothelial Growth Factor Avascular endothelial growth factor A, mouseCGRPDevelopmentDiaphragmTenomodulinVEGF-A

Identifiers

PMID39813008

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.