Advanced LC-IMS-MS Protocol for Holistic Metabolite Analysis in Wine and Grape Samples.

Vania Sáez, Sara Ferrero-Del-Teso, Fulvio Mattivi, Urska Vrhovsek, Panagiotis Arapitsas

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Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 authors.

Vania SáezDepartment of Food Quality and Nutrition, Research and Innovation Center, Fondazione Edmund Mach, San Michele all'Adige, Italy.

Sara Ferrero-Del-TesoInstituto de Ciencias de la Vid y del Vino (ICVV) (Universidad de La Rioja-CSIC-Gobierno de La Rioja), Logroño, La Rioja, Spain.

Fulvio MattiviDepartment of Food Quality and Nutrition, Research and Innovation Center, Fondazione Edmund Mach, San Michele all'Adige, Italy.

Urska VrhovsekDepartment of Food Quality and Nutrition, Research and Innovation Center, Fondazione Edmund Mach, San Michele all'Adige, Italy.

Panagiotis ArapitsasDepartment of Food Quality and Nutrition, Research and Innovation Center, Fondazione Edmund Mach, San Michele all'Adige, Italy. parapitsas@uniwa.gr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The final aim of metabolomics is the comprehensive and holistic study of the metabolome in biological samples. Therefore, the use of instruments that enable the analysis of metabolites belonging to various chemical classes in a wide range of concentrations is essential, without compromising on robustness, resolution, sensitivity, specificity, and metabolite annotation. These characteristics are crucial for the analysis of very complex samples, such as wine, whose metabolome is the result of the sum of metabolites derived from grapes, yeast(s), bacteria(s), and chemical or physical modification during winemaking. In recent years, a big advantage, in this direction, was the hardware developments on hyphenated instruments that enable the integration of liquid chromatography (LC), ion mobility spectrometry (IMS), and mass spectrometry (MS). This chapter describes an LC-IMS-MS protocol for the analysis of wine and grape samples as well as the use of IMS data in metabolite annotation.

Indexed as

Ion Mobility SpectrometryMass SpectrometryMetabolomicsVitisWineChromatography, LiquidMetabolomeCCSHDMSEMass spectrometryMetabolite annotationMetabolomicsTraveling wave ion mobilityVitis

Identifiers

PMID39812986

DOI10.1021/es5002105

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.