Evidence map›Paper›PMID 39808375›Full record

ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2025

High detection rate of parasitic load by qPCR targeting 18S rDNA in blood of patients with active leishmaniasis lesions.

Gabriel Victor Castanheira, Valdir Sabbaga Amato, Felipe Francisco Tuon, Letícia Ramos Dantas, Silvio Fernando Guimarães de Carvalho, Thallyta Maria Vieira, Ana Paula Venuto Moura, Jackeline Maria de Sousa Lima Lopes, Gustavo Henrique Johanson, Christini Takemi Emori and 1 more

Abstract readEvaluation Study
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In one paragraph

Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Gabriel Victor CastanheiraFaculdade de Medicina, Departamento de Infectologia E Medicina Tropical, Universidade de São Paulo, São Paulo, São Paulo, Brazil.
Valdir Sabbaga AmatoFaculdade de Medicina, Departamento de Infectologia E Medicina Tropical, Universidade de São Paulo, São Paulo, São Paulo, Brazil.
Felipe Francisco TuonLaboratório de Doenças Infecciosas E Emergentes, Pontifícia Universidade Católica Do Paraná, Rua Imaculada Conceição 1155, Curitiba, PR, 80215-901, Brazil. felipe.tuon@pucpr.br.
Letícia Ramos DantasLaboratório de Doenças Infecciosas E Emergentes, Pontifícia Universidade Católica Do Paraná, Rua Imaculada Conceição 1155, Curitiba, PR, 80215-901, Brazil.
Silvio Fernando Guimarães de CarvalhoDepartamento Saúde da Mulher E da Criança, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil.
Thallyta Maria VieiraDepartamento de Biologia Geral, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil.
Ana Paula Venuto MouraDepartamento de Biologia Geral, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil.
Jackeline Maria de Sousa Lima LopesUniversidade Estadual de Montes Claros, Programa de Pós-Graduação Em Ciências da Saúde, Montes Claros, Minas Gerais, Brazil.
Gustavo Henrique JohansonFaculdade de Medicina, Departamento de Infectologia E Medicina Tropical, Universidade de São Paulo, São Paulo, São Paulo, Brazil.
Christini Takemi EmoriFaculdade de Medicina, Departamento de Infectologia E Medicina Tropical, Universidade de São Paulo, São Paulo, São Paulo, Brazil.
Regina Maia de SouzaFaculdade de Medicina, Laboratório de Parasitologia, Instituto de Medicina Tropical, Universidade de São Paulo, São Paulo, Brazil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study aimed to standardize qPCR techniques using these molecular markers kDNA and 18S rDNA across three sample types: peripheral blood, guanidine-treated blood, and tissue. The secondary objective is to evaluate the performance of 18S rDNA target in samples from 46 patients with confirmed tegumentary leishmaniasis. After obtaining the standard curve from reference strains with Leishmania, qPCR curves were standardizations and the Cts results of the patient samples were described using abstract measures. Specific specification equations (EEG) with normal distribution and identity link function were constructed to compare each type of clinical sample. To identify the differences among samples and techniques, multiple comparisons with Bonferroni post-test was performed. The kDNA and 18S rDNA demonstrated high sensitivity, detecting as few as 10⁻

Indexed as

BloodDNA, RibosomalLeishmaniaLeishmaniasisLeishmaniasis, CutaneousParasite LoadReal-Time Polymerase Chain ReactionRNA, Ribosomal, 18SAdultDNA, ProtozoanFemaleHumansMaleMiddle AgedSensitivity and SpecificityYoung AdultDNA, ProtozoanDNA, RibosomalRNA, Ribosomal, 18SCutaneous leishmaniasisLeishmaniaMolecular diagnosisReal time PCR

Identifiers

PMID39808375

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.