ArticleAmerican journal of botany2025
Rapid detection of RNase-based self-incompatibility in Lysimachia monelli (Primulaceae).
Article in American journal of botany, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- A lectin receptor-like kinase controls self-pollen recognition inProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Linking environmental adaptation and genetic structure to the biogeography of flower colour lineages in Lysimachia monelli (Primulaceae).Plant biology (Stuttgart, Germany) · 2025Article
- The genetic architecture of quantitative variation in the self-incompatibility response within Phlox drummondii (Polemoniaceae).Genetics · 2025Article
- S-RNase-based self-incompatibility in angiosperms: Degradation, condensation, and evolution.Plant physiology · 2025Review
- Rapid detection of RNase-based self-incompatibility in Lysimachia monelli (Primulaceae).American journal of botany · 2025Article
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7 authors.
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Abstract
premisePrimroses famously employ a system that simultaneously expresses distyly and filters out self-pollen. Other species in the Primulaceae family, including Lysimachia monelli (blue pimpernel), also express self-incompatibility (SI), but involving a system with distinct features and an unknown molecular genetic basis.
methodsWe utilize a candidate-based transcriptome sequencing (RNA-seq) approach, relying on candidate T2/S-RNase Class III and S-linked F-box-motif-containing genes and harnessing the unusual evolutionary and genetic features of SI, to examine whether an RNase-based mechanism underlies SI in L. monelli. We term this approach "SI detection with RNA-seq" (SIDR).
resultsThe results of sequencing, crossing, population genetics, and molecular evolutionary features each support a causal association linking the recovered genotypes with SI phenotypes. The finding of RNase-based SI in Primulaceae (Ericales) all but cements the long-held view that this mechanism was present in the ancestral pentapetal eudicot, whose descendants now comprise two-thirds of angiosperms. It also significantly narrows the plausible maximum age for the heterostyly evolution within the family.
conclusionsSIDR is powerful, flexible, inexpensive, and most critically enables work in often-neglected species. It may be used with or without candidate genes to close enormous gaps in understanding the genetic basis of SI and the history of breeding system evolution.
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