A Prototype of Ultrasensitive Time-Resolved Fluoroimmunoassay with Enhanced Fluorescence System for the Trace Determination of Urinary 8-Hydroxy-2`-Deoxyguanosine, the DNA Oxidative Stress Biomarker.

Ibrahim A Darwish, Daohong Zhang, Ayman M Al-Qaaneh, Waleed Alahmad

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Article in Journal of fluorescence, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 authors.

Ibrahim A DarwishDepartment of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh, 11451, Saudi Arabia. idarwish@ksu.edu.sa.

Daohong ZhangCollege of Food Engineering, Ludong University, Yantai, 264025, Shandong, China.

Ayman M Al-QaanehDepartment of Allied Health Sciences, Al-Balqa Applied University (BAU), Al-Salt, 19117, Jordan.

Waleed AlahmadDepartment of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok, Phayathai Road Pathumwan, 10330, Thailand.

Funding

The researchers Supporting Project, King Saud University, Riyadh RSPD2025R944

6 · The paper itself

Abstract

This study presents a new highly sensitive and specific time-resolved fluoroimmunoassay (TRFIA) for the measurement of trace amounts of the urinary 8-hydroxy-2`-deoxyguanosine (8-OHdG) which is a biomarker for oxidative stress on DNA. The assay relied on a competitive binding approach and a mouse monoclonal antibody which recognized 8-OHdG with high specificity. In this assay, 8-OHdG conjugated with bovine serum albumin protein (8-OHdG-BSA) was employed as a solid phase antigen. The competition occurred between the 8-OHdG present in the sample solutions and the assay plate-coated 8-OHdG-BSA for a limited quantity of the anti-8-OHdG antibody labeled with a chelate of europium of ethylenediaminetetraacetic acid. The fluorescence signal of the europium chelate-labeled antibody was enhanced by a solution composed of thenoyltrifluoroacetone, trioctylphosphine oxide, and Triton X-100. The validation demonstrated a working range of 10-600 pg mL

Indexed as

DeoxyguanosineDNAOxidative Stress8-Hydroxy-2'-DeoxyguanosineAnimalsBiomarkersCattleFluorescenceFluoroimmunoassayHumansLimit of DetectionMiceSerum Albumin, BovineTime Factors8-Hydroxy-2'-DeoxyguanosineBiomarkersDeoxyguanosineDNASerum Albumin, Bovine8-Hydroxy-2’-deoxyguanosineBiomarkersDNA stress damageTime-resolved fluoroimmunoassayUrine

Identifiers

PMID39806235

DOI10.1038/srep32581

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.