Evidence map›Paper›PMID 39806115›Full record

ArticleMikrochimica acta2025

Amplification-free sensitive detection of Staphylococcus aureus by spherical nucleic acid triggered CRISPR/Cas12a and Poly T-Cu reporter.

Xiaoyu Zhang, Ruimeng Sun, Haoran Zheng, Yanfei Qi

Abstract read
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In one paragraph

Article in Mikrochimica acta, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Xiaoyu ZhangSchool of Public Health, Jilin University, Changchun, Jilin, 130021, P. R. China.
Ruimeng SunSchool of Public Health, Jilin University, Changchun, Jilin, 130021, P. R. China.
Haoran ZhengSchool of Public Health, Jilin University, Changchun, Jilin, 130021, P. R. China.
Yanfei QiSchool of Public Health, Jilin University, Changchun, Jilin, 130021, P. R. China. qiyanfei@jlu.edu.cn.

Funding

National Natural Science Foundation of China 82073602Natural Science Foundation of Jilin Province 20200201081JC
6 · The paper itself

Abstract

A spherical nucleic acid (SNA, AuNPs-aptamer) into CRISPR/Cas12a system combined with poly T-template copper nanoparticles as fluorescence reporter was fabricated to establish an amplification-free sensitive method for Staphylococcus aureus (S. aureus) detection. This method, named PTCas12a, utilizes the concept that the bifunction of SNA recognizes the S. aureus and triggers the Cas12a cleavage activity. Then, the Cas12a enzyme cleaves the Poly T40 to generate a signal change in Poly T-Cu fluorescence, indicating the presence or absence of the target bacteria. The PTCas12a platform demonstrated a detection limit as low as 3.0 CFU/mL (3 N/S) in a wide response range of 1.0 × 10

Indexed as

Biosensing TechniquesCRISPR-Associated ProteinsCRISPR-Cas SystemsEndodeoxyribonucleasesStaphylococcus aureusAptamers, NucleotideBacterial ProteinsCopperFluorescent DyesGoldLimit of DetectionMetal NanoparticlesAptamers, NucleotideBacterial ProteinsCas12a proteinCopperCRISPR-Associated ProteinsEndodeoxyribonucleasesFluorescent DyesGoldCRISPR/Cas12aFluorescence detectionFood-borne pathogenSpherical nucleic acid

Identifiers

PMID39806115

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.