ArticleScientific reports2025
High throughput method for simultaneous screening of membrane permeability and toxicity for discovery of new cryoprotective agents.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Multi-omics and developmental comparison of direct and conventional warming methods in vitrified human and mouse cleavage-stage embryos.Human reproduction (Oxford, England) · 2026Article
- Molecular Mechanisms of Basil (Molecules (Basel, Switzerland) · 2026Article
- A High-Throughput Platform for Measuring and Predicting Vitrification Behavior in Multicomponent Aqueous Solutions.ACS applied materials & interfaces · 2026Article
- A high throughput platform for measuring and predicting vitrification behavior in multicomponent aqueous solutions.bioRxiv : the preprint server for biology · 2026Article
- Comparison of clinical therapeutic effects between high-flux dialysis and low-flux dialysis.American journal of translational research · 2026Article
- Thermodynamic Principles of Emerging Cryopreservation Technologies.Methods in molecular biology (Clifton, N.J.) · 2026Review
- Article
- High-throughput evaluation of cryoprotective agents for mixture effects that reduce toxicity.Cryobiology · 2025Article
- Structural and Viability Assessment of Bovine Corneas Preserved at an Accessible Low-Temperature (-20 °C) for Eye Irritation Models.ACS omega · 2025Article
- A size-dependent ideal solution model for liquid-solid phase equilibria prediction in aqueous organic solutions.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
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4 authors.
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Abstract
Cryoprotective agent (CPA) toxicity is the most limiting factor impeding cryopreservation of critically needed tissues and organs for transplantation and medical research. This limitation is in part due to the challenge of rapidly screening compounds to identify candidate molecules that are highly membrane permeable and non-toxic at high concentrations. Such a combination would facilitate rapid CPA permeation throughout the sample, enabling ice-free cryopreservation with minimal toxicity. This study presents a new method for rapidly assessing the cell membrane permeability and toxicity of candidate CPAs. The new method enables ~ 100 times faster permeability measurement than previous methods, while also allowing assessment of CPA toxicity using the same 96-well plate. We tested five commonly used CPAs and 22 less common ones at both 4 °C and room temperature, with 23 of them passing the screening process based on their favorable toxicity and permeability properties. Considering its advantages such as high throughput measurement of membrane permeability along with simultaneous toxicity assessment, the presented method holds promise as an effective initial screening tool to identify new CPAs for cryopreservation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.