Evidence map›Paper›PMID 39804851›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2025

Scale-Up of Human Amniotic Epithelial Cells Through Regulation of Epithelial-Mesenchymal Plasticity Under Defined Conditions.

Wangping Hao, Yi Luo, Jia Tian, Yuefeng Lu, Yangyang Cui, Ying Zhang, Xiao Jin, Hongjuan Ye, Mengqi Lu, Jinjia Song and 3 more

Abstract read
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In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
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  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Wangping HaoInstitute for Regenerative Medicine, State Key Laboratory of Cardiology and Medical Innovation Center, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, 200123, P. R. China.
Yi LuoInstitute for Regenerative Medicine, State Key Laboratory of Cardiology and Medical Innovation Center, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, 200123, P. R. China.
Jia TianState Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, P. R. China.
Yuefeng LuShanghai iCELL Biotechnology Co., Ltd, Shanghai, 200335, P. R. China.
Yangyang CuiShanghai iCELL Biotechnology Co., Ltd, Shanghai, 200335, P. R. China.
Ying ZhangShanghai iCELL Biotechnology Co., Ltd, Shanghai, 200335, P. R. China.
Xiao JinShanghai iCELL Biotechnology Co., Ltd, Shanghai, 200335, P. R. China.
Hongjuan YeInstitute for Regenerative Medicine, State Key Laboratory of Cardiology and Medical Innovation Center, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, 200123, P. R. China.
Mengqi LuInstitute for Regenerative Medicine, State Key Laboratory of Cardiology and Medical Innovation Center, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, 200123, P. R. China.
Jinjia SongInstitute for Regenerative Medicine, State Key Laboratory of Cardiology and Medical Innovation Center, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, 200123, P. R. China.
Weiqing ZhouState Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, P. R. China.
Wencheng ZhangInstitute for Regenerative Medicine, State Key Laboratory of Cardiology and Medical Innovation Center, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, 200123, P. R. China.
Zhiying HeInstitute for Regenerative Medicine, State Key Laboratory of Cardiology and Medical Innovation Center, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, 200123, P. R. China.ORCID https://orcid.org/0000-0002-0087-2277

Funding

Major Program of National Key Research and Development Project 2020YFA0112600National Natural Science Foundation of China 82173019National Natural Science Foundation of China 82203741National Natural Science Foundation of China 82270638National Natural Science Foundation of China 82300718National Natural Science Foundation of China 82301904Peak Disciplines (Type IV) of Institutions of Higher Learning in ShanghaiProject of Shanghai Science and Technology Commission 22Y11908500Project of Shanghai Science and Technology Commission 22ZR1451100Shanghai Engineering Research Center of Stem Cells Translational Medicine 20DZ2255100Shanghai Pujiang Program 23PJ1422500
6 · The paper itself

Abstract

Human amniotic epithelial cells (hAECs) have shown excellent efficacy in clinical research and have prospective applications in the treatment of many diseases. However, the properties of the hAECs and their proliferative mechanisms remain unclear. Here, single-cell RNA sequencing (scRNA-seq) is performed on hAECs obtained from amniotic tissues at different gestational ages and passages during in vitro culture. The results showed that the proliferation of hAECs is associated with epithelial-mesenchymal plasticity (EMP) during amniogenesis. Freshly isolated, full-term hAECs are identified as mature epithelial cells. Once cultured in vitro, they are observed to rapidly undergo epithelial-mesenchymal transition (EMT) and enter a partial epithelial-mesenchymal transition (pEMT) state to regain their EMP properties and proliferation capacities. With the continuous development of EMT, hAECs eventually enter a senescent state. The addition of SB431542 and microcarrier screening enabled the effective 3D expansion of hAECs by 50 fold while maintaining the EMP status in hAECs for further proliferation. This study not only elucidated the central proliferation mechanism of hAECs during development and expansion but also optimized the in vitro culture system so that it is sufficient to generate hAECs for 50 patients from a single donor amniotic membrane.

Indexed as

AmnionEpithelial CellsEpithelial-Mesenchymal TransitionCell PlasticityCell ProliferationCells, CulturedFemaleHumans3D microcarrier expansionepithelial‐mesenchymal plasticity (EMP)human amniotic epithelial cells (hAECs)partial epithelial‐mesenchymal transition (pEMT)single‐cell RNA sequencing (scRNA‐seq)

Identifiers

PMID39804851
PMCPMC11923953

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.