Evidence map›Paper›PMID 39804547›Full record

ArticleTissue engineering and regenerative medicine2025

Rapid Video Analysis for Contraction Synchrony of Human Induced Pluripotent Stem Cells-Derived Cardiac Tissues.

Yuqing Jiang, Mingcheng Xue, Lu Ou, Huiquan Wu, Jianhui Yang, Wangzihan Zhang, Zhuomin Zhou, Qiang Gao, Bin Lin, Weiwei Kong and 2 more

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In one paragraph

Article in Tissue engineering and regenerative medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

12 authors.

Yuqing JiangPen-Tung Sah Institute of Micro-Nano Science and Technology, Xiamen University, Xiamen, 361102, Fujian, China.ORCID http://orcid.org/0009-0001-8420-9471
Mingcheng XuePen-Tung Sah Institute of Micro-Nano Science and Technology, Xiamen University, Xiamen, 361102, Fujian, China.
Lu OuPen-Tung Sah Institute of Micro-Nano Science and Technology, Xiamen University, Xiamen, 361102, Fujian, China.
Huiquan WuPen-Tung Sah Institute of Micro-Nano Science and Technology, Xiamen University, Xiamen, 361102, Fujian, China.
Jianhui YangPen-Tung Sah Institute of Micro-Nano Science and Technology, Xiamen University, Xiamen, 361102, Fujian, China.
Wangzihan ZhangPen-Tung Sah Institute of Micro-Nano Science and Technology, Xiamen University, Xiamen, 361102, Fujian, China.
Zhuomin ZhouPen-Tung Sah Institute of Micro-Nano Science and Technology, Xiamen University, Xiamen, 361102, Fujian, China.
Qiang GaoGuangdong Provincial People's Hospital, Guangzhou, 510080, Guangdong, China.
Bin LinGuangdong Beating Origin Regenerative Medicine Co. Ltd., Foshan, 528231, Guangdong, China.
Weiwei KongGuangdong Beating Origin Regenerative Medicine Co. Ltd., Foshan, 528231, Guangdong, China.
Songyue ChenPen-Tung Sah Institute of Micro-Nano Science and Technology, Xiamen University, Xiamen, 361102, Fujian, China. s.chen@xmu.edu.cn.ORCID http://orcid.org/0000-0003-1827-4969
Daoheng SunPen-Tung Sah Institute of Micro-Nano Science and Technology, Xiamen University, Xiamen, 361102, Fujian, China.ORCID http://orcid.org/0000-0001-7729-1481

Funding

Innovative Research Group Project of the National Natural Science Foundation of China No. U2005214the National Key Research and Development Program of China 2022YFB4600600
6 · The paper itself

Abstract

backgroundThe contraction behaviors of cardiomyocytes (CMs), especially contraction synchrony, are crucial factors reflecting their maturity and response to drugs. A wider field of view helps to observe more pronounced synchrony differences, but the accompanied greater computational load, requiring more computing power or longer computational time.

methodsWe proposed a method that directly correlates variations in optical field brightness with cardiac tissue contraction status (CVB method), based on principles from physics and photometry, for rapid video analysis in wide field of view to obtain contraction parameters, such as period and contraction propagation direction and speed.

resultsThrough video analysis of human induced pluripotent stem cell (hiPSC)-derived CMs labeled with green fluorescent protein (GFP) cultured on aligned and random nanofiber scaffolds, the CVB method was demonstrated to obtain contraction parameters and quantify the direction and speed of contraction within regions of interest (ROIs) in wide field of view. The CVB method required less computation time compared to one of the contour tracking methods, the Lucas-Kanade (LK) optical flow method, and provided better stability and accuracy in the results.

conclusionThis method has a smaller computational load, is less affected by motion blur and out-of-focus conditions, and provides a potential tool for accurate and rapid analysis of cardiac tissue contraction synchrony in wide field of view without the need for more powerful hardware.

Indexed as

Induced Pluripotent Stem CellsMyocardial ContractionMyocytes, CardiacVideo RecordingHumansHuman induced pluripotent stem cellsImage processingMyocardial contractionScaffold 3D cell cultureTissue engineering

Identifiers

PMID39804547
PMCPMC11794902

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